Monsanto Company

John H Johnson

Joseph E Fields

Lloyd E Weeks

Samuel S Asculai

Robert A Heimsch

Margaret J Reents

Stefan A Weiss

A concentrate of blood coagulation Factor VIII:C is obtained in high yield by fractionation of blood plasma with a sequence of adsorption steps employing two different water-insoluble, cross-linked polyelectrolyte copolymers, each in the presence of exogenous heparin.

Heparin polyelectrolyte polymer complex

Production of blood coagulation factor VIII:C

The disclosure relates to a method of producing an immune response in a warm-blooded animal comprising administering to said animal from about 0.1 to about 100 mg/Kg of body weight of a composition selected from the group consisting of a copolymer of olefin monomer and polycarboxylic anhydride having an average molecular weight of from about 300 to about 1500 in which said copolymer is derivatized to contain (a) half-amide, half-carboxyl acid groups and (b) imide groups in which said imide groups comprise from about 5% to about 40% by weight of said derivatized groups, and the pharmaceutically acceptable cationic salt derivatives of said derivatized copolymers.

Method of producing immune response by administering polymeric

The level of blood triglycerides is controlled by administration of polymers of unsaturated carboxylic acids, for example, copolymers of such acids with high molecular weight olefins or vinyl ethers.

Method for controlling blood triglycerides

The disclosure relates to copolymers of olefin monomers having from 2 to about 4 carbon atoms and .alpha.,.beta.-unsaturated polycarboxylic anhydrides having from 4 to about 6 carbon atoms, having an average molecular weight of from about 300 to about 1500, and derivatized to contain both a half-amide, half-carboxylate salt function and an imide function in which said imide function comprises from about 5% to about 40% of said derivatized function. The utility of these copolymers in the management of tumor therapy to prevent tumor recurrence or development of metastases by an immunoregulatory mechanism has been demonstrated in rats and mice.

Polymeric immunoregulatory agents containing half-amide/half

The invention involves a method of weight control in which polymers of unsaturated carboxylic acid derivatives are orally administered to interfere with assimilation of fat. Polymers which inhibit action of pancreatic lipase, especially cationic polymers such as dialkylaminoalkyl imides of alkene/maleic anhydride copolymers, are particularly useful.

Method for controlling obesity

Olefin/maleic acid copolymer is refined to pharmaceutical quality by use of dichloroethane to remove contaminants, as by precipitating from solution with dichloroethane.

Preparation of refined olefin/maleic acid copolymers

The level of blood cholesterol in a living animal body in which a state of hypercholesterolemia exists is lowered by orally administering to said animal body a pharmaceutically effective amount of a polymer, which is (1) polymerized unsaturated carboxylic acid or anhydride, or (2) a copolymer of (a) an unsaturated monomer having, for Example, 2 to 30 carbon atoms and, (b) an unsaturated carboxylic acid, anhydride or derivative thereof as exemplified by octadecene-l/maleic anhydride copolymer. Polymers having lipophilic properties are a preferred class. A typical dosage is an amount which represents in the range of from about 0.01 to about 5.0% of the diet.

Method for lowering blood cholesterol level

The level of blood cholesterol in a living animal body in which a state of hypercholesterolemia exists is lowered by orally administering to said animal body a pharmaceutically effective amount of a polymer, which is (1) polymerized unsaturated carboxylic acid or anhydride, or (2) a copolymer of (a) an unsaturated monomer having, for example, 2 to 30 carbon atoms and, (b) an unsaturated carboxylic acid, anhydride or derivative thereof as exemplified by octadecene-1/maleic anhydride copolymer. Polymers having lipophilic properties are a preferred class. A typical dosage is an amount which represents in the range of from about 0.01 to about 5.0% of the diet.

Composition for lowering blood cholesterol level

A method of determining triglycerides in biological fluids which comprises reacting a biological fluid sample with a lipolytic enzyme to liberate free glycerol, reacting said free glycerol with a coupled enzyme series in an oxygenated aqueous solution containing ATP, GK, .alpha.-GPDH, NAD and an electron transfer agent, and measuring the uptake of oxygen by the oxidation of the resulting NADH with an oxygen-sensing electrode.

Triglycerides determination method

A method of determining creatine phosphokinase activity in biological fluids comprising reacting a biological fluid sample with a coupled enzyme series in an oxygenated aqueous solution containing creatine phosphate, ADP, glucose, HK, G-6-PDH, NADP and an electron transfer agent and measuring the uptake of oxygen by the oxidation of the resulting NADPH with an oxygen-sensing electrode.

Creatine phosphokinase determination method

A method of culturing cells in which the cells are fixed on a polyacrylonitrile surface.

Method of cell culture on polyacrylonitrile surface

A method of determining lactate dehydrogenase activity in biological fluids comprising reacting a biological fluid sample with a coupled enzyme series in an oxygenated aqueous solution containing lactate, nicotinamide adenine dinucleotide and an electron transfer agent, and measuring the uptake of oxygen by the oxidation of the resulting NADH with an oxygen-sensing electrode.

Growing community of inventors

Kirkwood, MO, United States of America

John H Johnson