Sru Biosystems, Inc.

John Gerstenmeier, Iii

Lance Gavin Laing

Gangadhar Jogikalmath

Timothy Smith

A device for simultaneously dispensing a solution containing a sample to a test device and aspirating the sample from the device. In one embodiment the device is hand-held and includes a control mechanism, e.g., button for activating the dispensing and aspiration of the sample. The testing device may take the form of a multi-well plate with a plurality of wells arranged in rows and columns, with the bottom of the wells formed as a photonic crystal biosensor. The device can be configured with dispense and aspirate manifolds and associated dispense and aspirate ports which are positioned in all the wells along a row or column of wells so as to simultaneously aspirate and dispense solution to all the wells in a row or column of the multi-well plate.

Simultaneous aspirator and dispenser for multiwell plates and similar devices

A method of making a measurement of binding affinity of a sample comprises the steps of: introducing the sample into a well having a bottom formed as a photonic crystal biosensor, wherein a portion of the sample becomes bound to the biosensor; making a measurement of the change in the shift in peak wavelength value as a function of time (k) from the well as the sample is introduced into the well; simultaneously dispensing the sample to the well and aspirating the sample from the well and measuring the change in the shift in peak wavelength value as a function of time (k) during the simultaneous dispensing and aspirating, and calculating an equilibrium association constant or an equilibrium dissociation constant for the sample from the values of kand k.

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Cleveland Heights, OH, United States of America

John Gerstenmeier, Iii