E.i. Dupont De Nemours and Company

John G Williams

Louis G Rosanio

Scott V Tingey

Jan Antoni Rafalski

Kenneth J Livak

A method of extracting cellular components, e.g., nucleic acids, particularly DNA, from biological, especially solid tissue, samples, particularly from plants, is provided, whereby all of the steps can be performed in one vessel, and the entire process can be automated. A vessel suitable for this process is provided, as well as a system for the automated performance of the process steps.

Cellular component extraction apparatus and disposable vessel useful

A process is provided for detecting polymorphisms on the basis of nucleotide differences in random segments of the nucleic acid by performing a primer extension reaction on the nucleic acids and comparing the extension reaction products. The random nucleic acid segment can be amplified by first dissociating the extension product from the template and contacting the dissociated extension product with a primer under conditions such that an amplification extension product is synthesized using the dissociated extension product as a template. Differences in the extension product are useful as markers in constructing genetic maps and as markers to distinguish or identify individuals.

Process for detecting polymorphisms on the basis of nucleotide

A method of extracting cellular components, e.g., nucleic acids, particularly DNA, from biological, expecially solid tissue, samples, particularly from plants is provided, whereby all of the steps can be performed in one vessel, and the entire process can be automated. A vessel suitable for this process is provided, as well as a system for the automated performance of the process steps.

Growing community of inventors

Landenberg, PA, United States of America

John G Williams