University of Saskatchewan

Biostar, Inc.

John G Manns

Andrew A Potter

Gregg Patrick Adams

Roger A Pierson

New immunological carrier systems, DNA encoding the same, and the use of these systems, are disclosed. The carrier systems include chimeric proteins which include a leukotoxin polypeptide fused to one or more selected GnRH multimers which comprise at least one repeating GnRH decapeptide sequence, or at least one repeating unit of a sequence corresponding to at least one epitope of a selected GnRH molecule. Under the invention, the selected GnRH sequences may all be the same, or may correspond to different derivatives, analogues, variants or epitopes of GnRH so long as the GnRH sequences are capable of eliciting an immune response. The leukotoxin functions to increase the immunogenicity of the GnRH multimers fused thereto.

Polynucleotides encoding LKT 111

GnRH-leukotoxin chimeras

New immunological carrier systems, DNA encoding the same, and the use of these systems, are disclosed. The carrier systems include chimeric proteins which comprise a leukotoxin polypeptide fused to a selected GnRH multimer which consists essentially of at least one repeating GnRH decapeptide sequence, or at least one repeating unit of a sequence corresponding to at least one epitope of a selected GnRH molecule. Under the invention, the selected GnRH sequences may all be the same, or may correspond to different derivatives, analogues, variants or epitopes of GnRH so long as the GnRH sequences are capable of eliciting an immune response. The leukotoxin functions to increase the immunogenicity of the GnRH multimer fused thereto.

GNRH-leukotoxin chimeras

Ultrasonography together with computer analysis tools are used to obtain measurements of gonadal density and size in male livestock, and particular in immunologically castrated boars in a slaughterhouse. These measurements are compared with reference thresholds with the main purpose of determining whether the immunologically castrated boars have entered puberty. Boars are likely to be subject to meat tainting subsequent to the onset of puberty due to biological secretions which occur at that time. This method allows the majority of tainted meat to be screened out prior to delivery.

Growing community of inventors

Saskatoon, Canada

John G Manns