Boehringer Mannheim Gmbh

Roche Diagnostics Gmbh

Applied Research Systems Ars Holding N.v.

John G Hurrell

Lance Scott Kuhn

Grenville Arthur Robinson

Hans-Joachim Guder

Robert J McEnroe

Rebecca W Muddiman

Mary L Ochs

Mary E Brown

A diagnostic kit, method, and apparatus for electrochemically determining the presence or concentration of an analyte in a sample. A mixture is formed which includes the sample, an enzyme acceptor polypeptide, an enzyme donor polypeptide, and a labeled substrate. The enzyme donor polypeptide is capable of combining with the enzyme acceptor polypeptide to form an active enzyme complex. The formation of such the active enzyme complex is responsive to the presence or concentration of the analyte in the fluid sample. The active enzyme hydrolyzes the labeled substrate, resulting in the generation of an electroactive label, which can then be oxidized at the surface of an electrode. A current resulting from the oxidation of the electroactive compound can be measured and correlated to the concentration of the analyte in the sample.

Electrochemical enzyme assay

Determination of substrate of coenzyme requiring enzyme

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Carmel, IN, United States of America

John G Hurrell