Sigma-aldrich Co. LLC

John G Dapron

Handong Li

William Karl Kappel

Richard J Mehigh

Kenneth E Heuermann

Edward B Watson, Iii

Venkatappa Viswanatha

Vankatappa Viswanatha

Edward B Watson

Edward B Iii Watson

A metal chelating composition having the formula: wherein

Metal chelating compositions for protein purification, detection or binding

An affinity matrix for use in affinity based molecular pull down and immunoprecipitation procedures. The affinity matrix comprises a polymeric support, a dye attached to a fraction of the polymeric support to enable optical detection of the polymeric support, and an affinity ligand other than the dye attached to a fraction of the polymeric support for the capture of a molecule. Also provided is a method for the isolation of a biomolecule from an aqueous solution. The method comprises combining the aqueous solution with an affinity matrix comprising a polymeric support and separating the affinity matrix from the aqueous solution. A dye is attached to a fraction of the polymeric support which enables optical detection and monitoring of the affinity matrix and, accordingly, reduces the likelihood of the loss of affinity matrix during the separation step. In addition, an affinity ligand other than the dye is also attached to a fraction of the polymeric support for the capture of the biomolecule.

Affinity matrices with enhanced visibility for molecular pull-down and immunoprecipitation applications

A metal chelating composition having the formula:wherein

A high capacity assay platform capable of binding target molecules includes a substrate and a polymer matrix attached to the substrate. The polymer matrix comprises a plurality of polymer molecules where at least some of the polymer molecules are covalently attached directly to the substrate and at least some of which molecules are crosslinked to other polymer molecules. Some of the polymer molecules have at least one binding ligand covalently attached thereto, and the density of the polymer matrix on the substrate is at least 2 &mgr;g/cm .

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Oakville, MO, United States of America

John G Dapron