Bio-rad Laboratories, Inc.

Bio-rad Europe Gmbh

John Flanagan

Steven R Binder

Ravi Kaul

Michelle Delanoy

Roger P Walker

Daniel Laune

Audrey Arjomandi

Florian Salipante

Isabelle Batxelli-Molina

Jeannette Fareh-Michiel

Pascale Galea

Emilie Du Paty

François Rieunier

The present invention relates to an immunoassay for the detection ofspecific IgG, IgM and IgG/IgM antibodies in biological samples suspected of Lyme infection. The immunoassay can be performed via a standard immunoassay format or on an automated platform. In various embodiments, the immunoassay uses one or morespecific chimeric peptides VlsE-FlaB (designated pFlaB-mV), VlsE-ErpP (designated pErp59-mV), VlsE-P35 (designated pP35-mV) alone or in combination with one or more outer surface protein C (Osp C) types B or I, p58 and DbpA. Other aspects of the invention provide antigen/substrate combinations and compositions comprising combinations of the disclosed peptides and/or proteins for use in the immunoassays described herein.

immunoassays and materials therefor

The invention provides a method of diagnosing Non-Alcoholic Steatohepatitis (NASH) and/or the hepatic fibrosis status of a subject, especially a subject afflicted with Non-alcoholic fatty liver disease (NAFLD) or NASH, based on the level of only three or more particular biomarkers. The invention further provides a kit suitable for performing said method and the use of said method and methods of treating patients diagnosed in accordance with the disclosed methods.

Biomarker combinations to simultaneously evaluate non-alcoholic steatohepatitis and hepatic fibrosis status

Growing community of inventors

Hercules, CA, United States of America

John Flanagan