Oread Laboratories, Inc.

University of Kansas

John F Stobaugh

Arnold J Repta

Takeru Higuchi, Deceased

Craig E Lunte

Susan M Lunte

Larry A Sternson

Pierre M De Montigny

Barry L Hogan

Kenji W Higuchi, Co-executor

Sunil Kakodkar

An interface is provided for use with a multiple sample acquisition device and a capillary electrophoresis separation device. The interface includes a body member having a base and an upstanding wall defining a generally hollow interior well. The upstanding wall includes an inlet passageway and an outlet passageway. An inlet guide member has an axially extending guide passageway disposed coaxially with the inlet passageway. An outlet guide member has an axially extending guide passageway disposed coaxially with the outlet passageway. An inlet capillary tube has an outer diameter sized for being received by the axially extending passageway of the inlet guide member, and a fluid passageway through which analyte can flow. The inlet capillary tube also includes a first end disposed exteriorly of the well and a second end disposed within the well. An outlet capillary tube has an outer diameter sized for being received in the axially extending guide passageway of the outlet guide member, and a fluid passageway through which analyte can flow. The outlet capillary tube also includes a first end disposed within the well and a second end disposed exteriorly of the well.

On-line interface and valve for capillary electophoresis system

Primary amines, secondary amines and peptides are fluorometrically assayed using a fluorogenic derivatization reagent of the formula: ##STR1## where Z is an electron withdrawing group or a fused benzene ring, Y and Y', which are identical or different, are nitrogen or carbon and X is a halogen or other leaving group. The above fluorogenic reagent reacts with the primary amine, secondary amine or peptide to form a cyclic fluorescent adduct.

Method for assaying primary amines, secondary amines and peptides using

A method of assaying primary amines or cyanide is disclosed. The process is carried out by reacting a substituted or unsubstituted naphthalene-2,3-dicarboxaldehyde or o-phthalaldehyde, reactive with primary amines and cyanide, with cyanide ions and at least one primary amine to form an adduct amenable to fluorometric or electrochemical assaying techniques. The adduct is then fluorometrically or electrochemically assayed. Also disclosed are 1-cyano-benz[f]isoindole adducts.

Assaying method for primary amines using aromatic dialdehydes

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Lawrence, KS, United States of America

John F Stobaugh