Life Technologies Corporation

Tangen Biosciences, Inc.

Other

Tang Biosciences, Inc.

John F Davidson

Jonathan Marc Rothberg

Todd Rearick

Wolfgang Hinz

James M Bustillo

Kim L Johnson

John R Nobile

John Harris Leamon

Antoine Van Oijen

Jonathan M Schultz

Martin Huber

Mark James Milgrew

Kristopher Barbee

Theo Nikiforov

David Marran

Shifeng Li

Guobin Luo

Richard Whitaker

Melville Davey

Jonathan O Schultz

Robert H Schiestl

Jonathan M Rothberg

Peter H Bissinger

Bradley Hann

Antoine M Van Oijen

John Richard Noble

Johnathan Rothberg

A method of sequencing a polynucleotide strand can include providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand; successively exposing the polynucleotide strand to the flow of four different dNTPs according to a first predetermined ordering; and successively exposing the polynucleotide strand to the flow of four different dNTPs according to a second predetermined ordering, wherein the second predetermined ordering is different from the first predetermined ordering.

Alternative nucleotide flows in sequencing-by-synthesis methods

Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

Methods and apparatus including array of reaction chambers over array of chemFET sensors for measuring analytes

Embodiments disclose an apparatus and methods for biological sample processing enabling isolation and enrichment of microbial or pathogenic constituents from the sample. A vessel for sample containment and extraction is further disclosed for engagement with a transducer capable of efficient sample disruption and lysis. Together these components provide a convenient and inexpensive solution for rapid sample preparation compatible with downstream analysis techniques.

Apparatus and method for cell, spore, or virus capture and disruption

Integrated sensor arrays for biological and chemical analysis

ChemFET sensor array including overlying array of wells

The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.

Isothermal methods for amplifying nucleic acid samples

The disclosed embodiments related to an apparatus and methods for biological sample processing enabling isolation and concentration of microbial or pathogenic constituents from the sample. Sample may be obtained directly from a specimen container, such as a vacutainer, and processed directly without risk of user exposure. The disclosed methods and apparatus provide a convenient and inexpensive solution for rapid sample preparation compatible with downstream analysis techniques.

Apparatus and method for extracting pathogens from biological samples

In some embodiments, the disclosure relates generally to methods as well as related compositions, systems, kits and apparatus comprising linking proteins to target compounds and/or to locations of interest using tethers. For example, the tether can be used to link the protein to a target compound, for example, to link an enzyme to a substrate. Similarly, the tether can be used to link the protein at or near a desired location on a surface. In one group of embodiments, the tether includes a polynucleotide and the target compound or location on the surface includes another polynucleotide that is capable of hybridizing to the tether. In such embodiments, the tether can be used to link the protein to the target compound or location using nucleic acid hybridization.

Linking methods, compositions, systems, kits and apparatuses

Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

Modified proteins and methods of making and using same

Methods and apparatus for measuring analytes

Growing community of inventors

Guilford, CA, United States of America

John F Davidson