Cryolife, Inc.

John F Carpenter

Kelvin G Brockbank

Brenda S Morse

A Denise Turner

A method of revitalizing cells or tissues that are to be cryopreserved for storage at ultracold temperatures, e.g. -196.degree. C. is disclosed which comprises preincubation of the cells or tissue from about 5 minutes to about 24 hours. The preincubation may be conducted at a temperature ranging from about 27.degree. C. to about 42.degree. C., after which the tissue or cells are cryopreserved.

Process of revitalizing cells prior to cryopreservation

A method of revitalizing cells or tissues that are to be cryopresserved for storage at ultracold temperatures, e.g. -196.degree. C. is disclosed which comprises preincubation of the cells or tissue from about 5 minutes to about 24 hours. The preincubation may be conducted at a temperature ranging from about 27.degree. C. to 42.degree. C., after which the tissue or cells are cryopreserved.

Process of revitalizing cells and tissue prior to cryopreservation

A method is disclosed for preparing a transplantable tissue which has been cryopreserved with an intracellular cryoprotectant and then thawed. After thawing, the tissue is treated with a diluent (or eluent) solution to reduce the level of cryoprotectant in the cells to a substantially non-toxic level. This is conducted using a single dilution step. Cryoprotectants which are removable in this manner include dimethylsulfoxide, glycerol, propanediol and other compounds which penetrate the cells. The dilution step does not require end point titration and can be conducted over a time period greater than about 5 minutes.

Process for preparing tissue for transplantation

The present invention relates to a system for use in the preparation of autologous or single-donor fibrin sealant. The invention further relates to a method of preparing fibrin sealant.

Growing community of inventors

Marietta, GA, United States of America

John F Carpenter