The University of Texas System

Applied Biosystems, LLC

Lynx Therapeutics, Inc.

John E Wiktorowicz

Allan R Brasier

Hyunsu Ju

Yefim M Raysberg

Adrian Recinos

Heidi Spratt

William E Werner

David M Demorest

Nisha Jain Garg

Tadeusz Kochel

Nikos Vasilakis

Laura H V G Gil

Eduardo Nascimento

Ernesto E T Marquez

Josefina Nores Garcia

Marli Tenorio

The present invention provides compositions and methods for detecting, analyzing, and identifying biomolecules used to diagnose patient with risk of DHF. More particularly, the invention provides plasma biomarkers including complement factor D to complement factor H (FactorD/FactorH) ratio and levels of one or more of IL2, desmoplakin, and high molecular weight albumin, which are used to detect risk of developing DHF.

Method and biomarkers for the detection of dengue hemorrhagic fever

The present invention provides methods for detecting, analyzing, and identifying biomolecules used to identifying patient with dengue-like symptom who are at risk of DHF. The inventive method comprises detecting in a sample from a subject dengue infected patient one or more biomarkers selected from the group consisting of IL-10, fibrinogen, C4A, immunoglobulin, tropomyosin, and three isoforms of albumin, and which are used in a predictive MARS model to detect patients with risk of developing DHF.

Method for the detection of an albumin isoform

Certain embodiments include methods for assessing a subject having a trypanosome infection for the presence or absence of indications of cardiomyopathy.

Biomarkers for chagas disease related cardiomyopathy

Methods and biomarkers for the detection of dengue hemorrhagic fever

Disclosed are an apparatus, system, and method for two dimensional electrophoresis of analytes of interest, particularly polypeptides. The apparatus includes a sample separation cavity comprising (1) an electrophoresis region located along an upper portion of the cavity for performing charge and/or size-based electrophoresis in a first dimension along the upper portion, and (2) below the electrophoresis region, a second electrophoresis region for performing electrophoresis in a second dimension in a direction substantially perpendicular to the first dimension. In one preferred embodiment, the second electrophoresis region contains an isoelectric focusing region containing a continuous pKa gradient immobilized on at least one of the major opposing surfaces of the cavity.

Electrophoresis apparatus and method

Disclosed are an apparatus, system, and method for two dimensional electrophoresis of analytes of interest, particularly polypeptides. The apparatus includes a sample separation cavity comprising (1) an electrophoresis region located along an upper portion of the cavity for performing charge and/or size-based electrophoresis in a first dimension along the upper portion, and (2) below the first said electrophoresis region, a second electrophoresis region for performing electrophoresis in a second dimension in a direction substantially perpendicular to the first dimension. In one preferred embodiment, the second electrophoresis region contains an isoelectric focusing region containing a continuous pKa gradient immobilized on at least one of the major opposing surfaces of the cavity.

A capillary tube with a charged inner surface for use in capillary electrophoresis and methods. The tube is filled with an electrolyte solution containing 0.05-30% weight/weight of a hydrophilic polymer which is characterized by (A) a molecular weight of 20-5,000 kilodaltons, and (B) a charge between 0.01 and 1.0% as measured by the molar percent of charged monomer subunits to the total polymer subunits, where said charged monomer subunits have the charge opposite to the wall charge at a selected electrophoresis pH.

Capillary electrophoresis molecular weight separation of biomolecules

A method for achieving desired electroosmotic flow characteristics in a capillary tube having charged surface groups. An electrolyte solution containing a compound effective to stably alter the charge of the tube walls is drawn into and through the tube during while the electroosmotic flow rate in the tube is being monitored, until a desired electroosmotic flow rate is achieved. The method can be used to optimize electrophoretic separation of charged protein or nucleic acid species in a capillary tube, and to produce capillary tubes with desired charge density properties.

Growing community of inventors

San Jose, CA, United States of America

John E Wiktorowicz