The Upjohn Co.

Pharmacia & Upjohn Company

John E Mott

David P Brunner

Richard J Kirschner

Frances M Eckenrode

Robert L Garlick

Gary C Harbour

Stephen B Lyle

Che-Shen C Tomich

Eric R Olson

John G Hoogerheide

Disclosed are expression vectors useful as vectors in recombinant methods to facilitate expression of exogenous genes in E. coli. Specifically, the disclosed expression vector has the following elements in operable linkage: the PL6m promoter, the TAT32 ribosome binding site and a gene encoding a heterologous polypeptide, Also disclosed are E. coli host cells transformed with this expression vector.

Expression vector containing PL6M promoter and TAT32 ribosome binding

The present invention provides analogs of somatotropins wherein the asparagine residue corresponding to position 99 of a bovine somatotropin is replaced with a compound selected from the group consisting of proline, aspartic acid, glutamic acid, serine, glycine, serine-serine or serine-aspartic acid. Particularly preferred analogs are bovine somatotropin and their use to increase milk production.

Somatotropin analogs

The present invention is based upon the discovery that proteins made from genes that include the CD4 sequence in its cDNA can make additional polypeptides as a result of internal translation initiation. This invention is thus directed to DNA sequences which eliminate internal initiation expression in sCD4.

Eliminating internal initiation of soluble CD4 gene

CDNAS encoding somatotropin, expression vectors and hosts

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Kalamazoo, MI, United States of America

John E Mott