Washington State University

Other

John E Crock

Rodney Bruce Croteau

Mark R Wildung

Christopher L Steele

J&ouml;rg Bohlmann

Peggy G Lemaux

Sheila M Colby

Reinhard Jetter

A cDNA encoding (E)-&#x3b2;-farnesene synthase from peppermint () has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-&#x3b2;-farnesene synthase (SEQ ID NO:2), from peppermint (). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-&#x3b2;-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-&#x3b2;-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-&#x3b2;-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-&#x3b2;-famesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-&#x3b2;-farnesene synthase may be used to obtain expression or enhanced expression of (E)-&#x3b2;-famesene synthase in plants in order to enhance the production of (E)-&#x3b2;-farnesene, or may be otherwise employed for the regulation or expression of (E)-&#x3b2;-farnesene synthase, or the production of its product.

Isolation and bacterial expression of a sesquiterpene synthase cDNA clone from peppermint (Mentha x piperita, L.) that produces the aphid alarm pheromone (E)-&#x3b2;-farnesene

cDNAs encoding, E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase from Grand Fir ( ) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID No:12; SEQ ID No:19 and SEQ ID No:23) are provided which code for the expression of E-&agr;-bisabolene synthase (SEQ ID No:13), &dgr;-selinene synthase (SEQ ID No:20) and &ggr;-humulene synthase (SEQ ID No:24), respectively, from Grand Fir ( ). In other aspects, replicable recombinant cloning vehicles are provided which code for E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase, or for a base sequence sufficiently complementary to at least a portion of E-&agr;-bisabolene synthase, &dgr;-selinene synthase or &ggr;-humulene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding E-&agr;-bisabolene synthase, &dgr;-selinene synthase or ,&ggr;-humulene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant sesquiterpene syntheses that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase may be used to obtain expression or enhanced expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase in plants in order to enhance the production of sesquiterpenoids, or may be otherwise employed for the regulation or expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase, or the production of their products.

Sesquiterpene synthases from grand fir (Abies grandis), and methods of use

Germacrene C synthase genes from have been cloned and sequenced. Transgenic expression of germacrene C synthase in plants can result in beneficial and useful characteristics such as increased host resistance to pathogens and herbivores and altered flavor and odor profiles.

Germacrene C synthase gene of Lycopersicon esculentum

cDNAs encoding E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase from Grand Fir (,) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID No:12 and SEQ ID No:19 and SEQ ID No:23) are provided which code for the expression of E-&agr;-bisabolene synthase (SEQ ID No:13), &dgr;-selinene synthase (SEQ ID No:20) and &ggr;-humulene synthase (SEQ ID No: 24), respectively, from Grand Fir (,). In other aspects, replicable recombinant cloning vehicles are provided which code for E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase, or for a base sequence sufficiently complementary to at least a portion of E-&agr;-bisabolene synthase, &dgr;-selinene synthase or &ggr;-humulene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding E-&agr;-bisabolene synthase, &dgr;-selinene synthase or &ggr;-humulene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant sesquiterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase may be used to obtain expression or enhanced expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase in plants, or may be otherwise employed for the regulation or expression of E-&agr;-bisabolene synthase, &dgr;-selinene synthase and &ggr;-humulene synthase.

Gymnosperm nucleic acid molecules encoding sesquiterpene synthases and methods of use

A cDNA encoding (E)-&bgr;-farnesene synthase from peppermint (,) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO: 1) is provided which codes for the expression of (E)-&bgr;-farnesene synthase (SEQ ID NO:2), from peppermint (,). In other aspects replicable recombinant cloning vehicles are provided which code for (E)-&bgr;-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-&bgr;-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-&bgr;-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-&bgr;-farnesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-&bgr;-farnesene synthase may be used to obtain expression or enhanced expression of (E)-&bgr;-farnesene synthase in plants in order to enhance the production of (E)-&bgr;-farnesene, or may be otherwise employed for the regulation or expression of (E)-&bgr;-farnesene synthase, or the production of its product.

Isolation and bacterial expression of a sesquiterpene synthase cDNA clone from peppermint (mentha x piperita, L.) that produces the aphid alarm pheromone (E)-&bgr;-farnesene

A cDNA encoding (E)-.beta.-farnesene synthase from peppermint (Mentha piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-.beta.-farnesene synthase (SEQ ID NO:2), from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-.beta.-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-.beta.-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-.beta.-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-.beta.-farnesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-.beta.-farnesene synthase may be used to obtain expression or enhanced expression of (E)-.beta.-farnesene synthase in plants in order to enhance the production of (E)-.beta.-farnesene, or may be otherwise employed for the regulation or expression of (E)-.beta.-farnesene synthase, or the production of its product.

Growing community of inventors

Moscow, ID, United States of America

John E Crock