Quest International Flavors & Food Ingredients Company, Division of

Novo Nordisk A/s

Unilever Patent Holdings B.v.

Van Den Bergh Foods Co., Division of Conopco, Inc.

Quest International Flavors & Food Ingredients Co.

Unilever Plc

Quest International Flavors & Food Ingredients Co., Division of Indopco

John D Marugg

James T Henderson

Ebenezer R Vedamuthu

Peter A Vandenbergh

Cornelis T Verrips

Adrianus M Ledeboer

Pieter D VanWassenaar

Ole Hvilsted Olsen

Dorrit A Aaslyng

Sven Branner

Sven Hastrup

Cornelis Johannes Leenhouts

Johan Haverkamp

Maarten Robert Egmond

Eric Casteleijn

Leif No slashedrskov-Lauritsen

Merete Simonsen

Pieter Dirk Van Wassenaar

Walter M Verhue

Maria Y Toonen

Arnoldus Theodorus Mooren

The present invention relates to modified subtilisins comprising a mutation in an amino acid sequence of a subtilisin at a position selected from the group consisting of 1, 2, 3, 4, 6, 9, 10, 12, 14, 15, 17, 18, 19, 20, 21, 22, 24, 25, 27, 36, 37, 38, 40, 41, 43, 44, 45, 46, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 75, 76, 77, 78, 79, 87, 89, 91, 94, 97, 98, 99, 100, 101, 103, 104, 105, 106, 107, 108, 109, 112, 113, 115, 116, 117, 118, 120, 126, 128, 129, 130, 131, 133, 134, 136, 137, 140, 141, 143, 144, 145, 146, 155, 156, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 170, 171, 172, 173, 181, 182, 183, 184, 185, 186, 188, 189, 191, 192, 194, 195, 197, 204, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 259, 260, 261, 262, 263, 265, 269, 271, 272 and 275. The present invention also relates to detergent compositions comprising a modified subtilisin of the present invention.

Modified subtilisins and detergent compositions containing same

A food-grade vector is provided which is suitable for transforming a food-grade host cell and is incapable of replicating autonomously in the host cell due to deletion of the replicase gene.

Food-grade vector suitable for transforming a food-grade host cell use

The invention relates to a process for the production of .alpha.-acetolactate and/or diacetyl, wherein a recombinant micro-organism containing an .alpha.-acetolactate synthase-encoding sequence is incubated in a medium containing an .alpha.-acetolactate precursor. The invention also provides a recombinant vector comprising a nucleotide sequence coding for an enzyme, which vector upon transfer into a host micro-organism enables expression of the nucleotide sequence in the host micro-organism, wherein the enzyme has .alpha.-acetolactate synthase activity.

Process for the preparation of .alpha.-acetolactic acid

Isolation and identification of a gene encoding for a bacteriocin precursor in Pediococcus acidilactici, cloning of the gene in a vector plasmid and transformation to bacteria is described. The bacteriocin is particularly useful for inhibiting Listeria in food products.

Cloned gene encoding for bacteriocin from Pediococcus acidilactici

A bacteriocin or polypeptide (LL-2 SEQ ID NO:2) derived from Lactococcus lactis subspecies lactis NRRL-B-18809 is described. Sequenced DNA and polypeptide precursor encoded thereby (SEQ ID NO: 1) are described. Methods of use and production of the polypeptide LL-2 are described.

Bacteriocin from lactococcus lactis subspecies lactis

A bacteriocin or polypeptide (LL-2 SEQ ID NO:2) derived from Lactococcus lactis subspecies lactis NRRL-B-18809 is described. Sequenced DNA and polypeptide precursor encoded thereby (SEQ ID NO: 1) are described Methods of use and production of the polypeptide LL-2 are described.

Cloned gene encoding for bacteriocin from pediococcus acidilactici

Bacteriocin from Lactococcus lactis subspecies lactis

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Utrecht, Netherlands

John D Marugg