Applied Biosystems, LLC

M.u. Engineering & Manufacturing Inc.

M. U. Engineering & Mfg., Inc.

John D Lytle

Michael W Hunkapiller

Charles R Connell

John A Bridgham

James R Skinner

William J Mordan

A real-time, automated, nucleic acid sequencing apparatus that offers high speed, definitive sequencing on many samples at the same time. The apparatus permits more than one clone to be sequenced at a time, thus vastly decreasing the time required to sequence longer fragments and reducing sequencing costs accordingly. The apparatus detects electromagnetic radiation from a plurality of lanes in an electrophoresis system wherein the plurality of lanes are arranged in a planar array. The apparatus includes an optical system for detecting the radiation at a plurality of wavelengths and is made up of a collection element, a filter for selectively transmitting the plurality of wavelengths received from the collection element, and a detection system for measuring intensity of the radiation received from the filter means. A translational stage is used for mounting the optical system and for moving the optical system parallel to the planar array in order to move the collection element back and forth across the lanes in order to receive radiation from the lanes, one lane at a time during electrophoresis. Also included is a computer system for controlling the filter and the stage, and for receiving intensity data from the detector and correlating that data with the corresponding lane and corresponding wavelengths transmitted by the filter in substantially real time.

Real time scanning electrophoresis apparatus for DNA sequencing

Lens cell for carrying a lens element of the type having spaced apart parallel planar registration faces on opposite ends thereof and at least one sidewardly extending tab, and comprising a cell body having a cylindrical wall defining an optical axis for the lens element. The cylindrical wall is formed with an annular inwardly extending protrusion providing an annular planar lens element seating surface facing one end of the cell body, the cylindrical wall has at least one slot formed therein and opening through said one end thereof whereby the lens element can be inserted into the cell body with one registration face in engagement with the annular seating surface and with its tab registered in said slot. A retainer is secured to said cell body and has an annular seating surface forming a registration surface for the other registration face of the lens element.

Lens cell

An all plastic lens assembly having a lens cell or housing and at least four separate airspaced lens elements mounted in the cell or housing, the lens elements are formed of plastic and at least one of the lens elements has an aspheric surface.

All plastic projection lens assembly with aspheric surface

Growing community of inventors

San Jose, CA, United States of America

John D Lytle