Allentown, LLC

Charles River Laboratories International, Inc.

John Coiro

Steve Miller

Brian Bilecki

Thomas P Schupsky

Alastair Maclennan, Iii

Kenneth S Henderson

Brian M Bilecki

Steven J Miller

Alastair Iii Maclennan

Embodiments of the present disclosure are directed to systems and methods for collection and analysis of environmental air dust (EAD) within an individually ventilated cage rack (IVR) environment for detecting pathogens. The method includes collection of an EAD sample by a collection media, isolation of a plurality of nucleic acids (e.g., RNA and/or DNA) representative of one or more infectious agents from the EAD sample, optional reverse transcription of RNA to cDNA if the isolated nucleic acids contain RNA, amplification of the cDNA and/or DNA (e.g., by polymerase chain reaction (PCR)), and assay interpretation. Optionally, the EAD sample may be analyzed with one or more other sample types (e.g., fecal pellets, oral swabs, body swabs, tissue, etc.) to improve detection of low-copy organisms.

Detection of infectious agents from environmental air dust

A disposable animal cage which can be used in a ventilated or static caging rack system includes a cage top and a cage base. Any corner or edge present in the interior of the cage base or the cage top that is accessible to an animal that can be contained in the cage is defined by surfaces having a conical section which is non-circular to prevent gnawing of the cage. A perforation section including a plurality of small apertures adjacent to one another can be formed in the cage for ventilation and prevention of gnawing by the animals.

Disposable animal cage for use in ventilated rack

A collection system for monitoring conditions of a rack of animal cages. An animal caging system including a plurality of animal cages removably connected to an exhaust. A collection media cartridge is slidably received within an opening in a collection media housing dock. The collection media housing dock is positioned between an exhaust plenum of the rack an exhaust air inlet unit. Collection media is removably received in the collection media cartridge. Exhaust air dust clings to the collection media as exhaust from the animal cages flows along the length of the collection media. The collection media cartridge can be removed from the collection media housing dock and the collection media can be removed for sampling the exhaust.

Method and system for monitoring air flow impurity

A method and system for monitoring conditions of an animal cage. An animal caging system including a plurality of animal cages removably connected to an exhaust. Cage collection media is removably mounted in a cage media housing coupled to an exhaust port of the animal cage. Exhaust air dust clings to the collection media as exhaust from the animal cages flows along the length of the collection media.

Method and system for monitoring air flow impurity within an animal cage

A disposable animal cage which can be used in a ventilated or static caging rack system includes a cage top and a cage base. Any corner or edge present in the interior of the cage base or the cage top that is accessible to an animal that can be contained in the cage is defined by surfaces having a conical section which is non-circular to prevent gnawing of the cage. The cage top can include a water housing mount pocket. The water housing mount pocket can have a conical shape to prevent an animal from gnawing the water housing mount pocket and a container received in the water housing mount pocket. A feed tray can be attached beneath a filter in a ceiling of the cage top to prevent an animal from gnawing the filter.

A method and system for monitoring conditions of a rack of animal cages. An animal caging system including a plurality of animal cages removably connected to an exhaust. Collection media is removably mounted in an exhaust plenum of the rack using a holder. Exhaust air dust clings to the collection media as exhaust from the animal cages flows along the length of the collection media. A door to the exhaust plenum can be opened and the collection media can be removed from the holder.

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Princeton, NJ, United States of America

John Coiro