Ge Healthcare Bio-sciences Ab

John Clachan

Andreas Axen

Jean-Luc Maloisel

Lars C Andersson

Andreas Axén

Camilla Estmer-Nilsson

Lena Maria Sandberg

Tesfai Gebru

Inger Andersson

Helena Lindgren

Anne Catharina Bergh

Tania Ahmad

Peder Bergvall

The present invention relates to a method of preparing a separation matrix comprising at least one insoluble carrier to which sulphate ligands have been attached via extenders, which method comprises coupling, in a first step, of the sulphate ligands to the extenders and, in a subsequent step, attaching the extenders to an insoluble carrier. The invention also relates to a separation matrix comprised of at least one insoluble carrier to which sulphate ligands have been attached via extenders. Advantageously, no sulphate ligands are directly attached to the insoluble carrier. The extenders may be natural polymers, such as dextran, and the insoluble carrier may be made from natural polymers, such as agarose, or synthetic polymers. The invention also relates to a method of purifying virus, such as influenza virus, using a separation matrix according to the invention.

Separation matrix for viral purification

The present invention relates to a method for generating at least one polydentate metal chelating affinity ligand, which method comprises the steps of

Method for generating metal chelating affinity ligands

The present invention relates to the preparation of a metal chelating separation medium, which comprises providing a compound defined by formula: R&#x2014;O&#x2014;CO&#x2014;(CH)&#x2014;CHNH&#x2014;CO&#x2014;OH wherein R is a selectively cleavable group; and n is an integer of 1&#x2013;4; reacting it with ethyl alcohol; derivatisation; cleavage of R; immobilisation of the resulting compound to a base matrix; and deprotection of the carboxylic acid. In one embodiment, the compound provided in the first step is ethyl ester of aspartic acid or of glutamic acid, and the derivatisation agent is bromo-acetic acid ethyl acetate.

Preparation of a metal chelating separation medium

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Uppsala, Sweden

John Clachan