Pierce Biotechnology, Inc.

Thermo Fisher Scientific Gmbh

John Charles Rogers

Joel Louette

Scott M Peterman

Bhavinkumar Patel

Bruno Domon

Elodie Duriez

Ryan Daniel Bomgarden

Eric Leigh Hommema

Greg Hermanson

Christopher Laurence Etienne

Penny JoAnn Jensen

Derek Karl Baerenwald

Bhavinkumar B Patel

This disclosure relates to the field of mass spectrometry analysis. In some embodiments, the disclosure relates to compositions and methods for detecting and quantifying proteins in the AKT-mTOR pathway by immunoprecipitation enrichment followed by mass spectrometry analysis.

Detection and quantification of AKT-mTOR pathway proteins

Quantitation of analytes, including but not limited to peptides, polypeptides, and proteins, in mass spectrometry using a labeled peptide coupled to a reporter, and a universal reporter.

Analyte mass spectrometry quantitation using a universal reporter

Methods and sets of non-biological reagents (elution reagents, tag isomers, tag reactive groups, crosslinkers) for single or multiplexed capture and gentle elution of biomolecules. Examples are provided using amine- and cysteine-reactive reagents for enrichment of proteins, peptides, and rare peptide modifications.

Methods and reagents for biomolecule labeling, enrichment and gentle elution

A method for absolute protein or peptide quantitation by mass spectroscopy. A sample containing a protein or peptide of interest is prepared for mass spectroscopy analysis. The sample is subjected to mass spectroscopy analysis at low resolution whereby a single additive mass spectroscopy peak is obtained, then is subjected to high resolution mass spectroscopy analysis whereby a plurality of mass spectroscopy peaks are obtained. The intensity of each of the plurality of mass spectroscopy peaks is quantitated either by comparison to an internal standard set, or by using a standard curve generated for each isotopologue set. Quantitation using a standard curve enhances quantitation across a dynamic range of analyte.

Absolute quantitation of proteins and protein modifications by mass spectrometry with multiplexed internal standards

Methods for producing an isotope-labeled mammalian, including a human, biomolecule, such as polypeptides and proteins, in a cell-free protein synthesis system. A biomolecule standard is produced having at least one isotope different in abundance than that of the naturally occurring isotopes in the biomolecule. Methods for quantifying biomolecules standards expressed using mammalian cell-free extracts are disclosed. Methods for producing such standards, kits, systems and reagents, relating to the use of isotope-labeled biomolecule as quantification standards in mass spectrometric and nuclear magnetic resonance analysis.

Methods for isotopically labeling biomolecules using mammalian cell-free extracts

Growing community of inventors

Rockton, IL, United States of America

John Charles Rogers