Millipore Corporation

Emd Millipore Corporation

John Charkoudian

Neil P Soice

Wilson Moya

Nanying Bian

Philip M Goddard

James Hamzik

Chen Wang

Jad Jaber

Joaquin A Umana

Nripen Singh

Yu Zhang

Arezki Boudif

Dave Brewster

Antoni Peters

Anja Dedeo

John Paul Puglia

Louis M Scarmoutzos

Anthony E Allegrezza, Jr

David Wang

Malcolm G Pluskal

Charles Phoebe

Nicole Fox

Nicole Vayo

John P Puglia

The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules.

Stimulus responsive polymers for the purification of biomolecules

The present invention relates to a nanofilter formed by using a porous ultrafiltration membrane as a precursor, and carefully controlling reaction conditions so as to maintain sufficient hydrophilic nature of the membrane while causing the pore structure to close to a nanofilter range (less than 400 Daltons). This produces a solvent stable cellulose nanofiltration membrane capable of operating at satisfactory flux in aqueous solutions, and being low binding to organic biomaterials.

Crosslinked cellulosic nanofiltration membranes

Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use.

Hydrophilic, high protein binding, low fluorescence, western blotting membrane

The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.

Affinity chromatography matrices and methods of making and using the same

This invention relates to a general method to surface modify porous membranes with a free radical polymerized polymer coating in a layer which is less than the thickness of the membrane. Another object of this invention relates to a general method to surface modify porous membranes with a free radical polymerized polymer coating to a predetermined and controlled depth from a first facial surface. Another object of this invention is to provide a general method to surface modify porous membranes with a free radical polymerized polymer coating into a pattern on one or both facial surfaces, the pattern maintaining its form to a controlled depth into the membrane. Membrane modification is spatially controlled through the use of a polymerization preventing agent (PPA), which prevents modification from occurring in defined spatially volumes of the membrane. The invention relates to porous membranes surface modified by the methods described.

Spatially-controlled modified porous membrane

The present invention provides porous media or membranes having a surface coating that includes a cross-linked terpolymer which has a superior combination of properties, including heat stable biomolecule resistant adsorptive properties, resistance to strong alkaline solutions, and low levels of extractable matter. In some preferred embodiments, the porous media is a porous membrane.

Coated membranes and other articles

The present invention provides porous media or membranes having a surface coating that includes a first coating of a cross-linked terpolymer and a second coating comprising a copolymer or a terpolymer modified with a hydrophilic or hydrophobic functional group which has a superior combination of properties, including heat stable biomolecule resistant adsorptive properties, resistance to strong alkaline solutions, and low levels of extractable matter.

Microporous membrane substrate having caustic stable, low protein binding surface

A porous membrane, such as a microporous or ultrafiltration membrane, is formed from a solution of a polysulfone and a free radical polymerizable monomer exposed to ultraviolet light to form a blend of the polysulfone and copolymers of polysulfone activation products and polymerized monomer having polymer segments covalently bonded to each other. The solution is separated into two phases including a porous solid phase comprising the membrane.

Polysulfone copolymer membranes and process

A polysulfone composition is formed from a solution of a polysulfone and a free radical polymerizable monomer exposed to ultraviolet light to form a blend of the polysulfone and copolymers of activated polysulfone and polymerized monomer having polymer segments covalently bonded to each other. The blend is separated from solvent of the solution to form a solid comprising the blend throughout its bulk matrix.

Growing community of inventors

Carlisle, MA, United States of America

John Charkoudian