Albert Einstein College of Medicine

John Chan

William R Jacobs, Jr

Paul Theodore Hamilton

Barry R Bloom

Michael C Little

Steven A Porcelli

Tsungda Hsu

Dee Dao

Stewart Jurgensen

Paul Riska

Kari Sweeney

Provided are mycobacteria deleted in at least a portion of a region 3 ESAT-6-like gene cluster. Also provided are mycobacteria comprising a mutation in an roc-1 gene. Additionally, vaccines comprising these mycobacteria are provided. Further provided are methods of making a recombinant, methods of inducing an immune response in a mammal, methods of inhibiting IL-12 production in a mammal, and methods of stimulating IL-12 production in a mammal. Vaccine adjuvants are also provided, as are methods of inducing immunity to a target antigen in a mammal.

Mycobacterial mutants inducing IL-12

Methods for using reporter mycobacteriophage (RM) and p-nitro-.alpha.-acetylamino-.beta.-hydroxy-propiophenone (NAP) to identify TB complex mycobacteria and distinguish these species from MOTT. RM-infected MOTT show little or no reduction in signal when treated with NAP. In contrast, TB complex mycobacteria infected with RM are distinguishable from RM-infected MOTT by a reduction in signal with NAP treatment.

Identification of mycobacterium tuberculosis complex species

Growing community of inventors

Hastings-on-Hudson, NY, United States of America

John Chan