Cornell Research Foundation Inc.

Other

Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Duke University

Sanford Scientific, Inc.

Greatbatch Gen-aid, Ltd.

Cornell University

John C Sanford

Stephen Albert Johnston

Donald K Ourecky

Edward D Wolf

Nelson K Allen

Kevin E Maloney

Dwight T Tomes

Theodore Mitchell Klein

Arthur Weissinger

Jack E Reich

Wilson Greatbatch

Alan D Blowers

Courtney A Weber

Joyce Van Eck

Franzine Smith

Tau-San Chou

R Sanders Williams

Robert B Eisenreich

Ronald Frank Bruner

Michael J DeVit

Joe Celeste

Dale J Loomis

Sandra G McElligott

Jack Reich, Ii

George L Slate, Deceased

A method of transferring a gene to vertebrate cells is disclosed. The method comprises the steps of: (a) providing microprojectiles, the microprojectiles carrying polynucleic acid sequences, the sequences comprising, in the 5' to 3&#x2032; direction, a regulatory sequence operable in the tissue cells and a gene positioned downstream of the regulatory sequence and under the transcriptional control thereof; and (b) accelerating the microprojectiles at the cells, with the microprojectiles contacting the cells at a speed sufficient to penetrate the cells and deposit the polynucleic acid sequences therein. Preferably, the target cells reside in situ in the animal subject when they are transformed. Preferred target cells are dermis or hypodermis cells, and preferred genes for insertion into the target cells are genes which code for proteins or peptides which produce a physiological response in the animal subject.

Particle-mediated transformation of vertebrate tissue cells

The present invention relates to a process for inducing a protective immune response in a mammal against a virus, in which a construct including a promoter and a DNA sequence encoding a protein or peptide producing an immune response against the virus is introduced into muscle or skin of the mammal via a microprojectile. Sufficient amounts of the construct are administered so as to produce a protective immune response in the mammal.

Induction of a protective immune response through microprojectiles coated with a DNA sequence encoding an immunogenic protein

Since its introduction into North America over 170 years ago, the poinsettia has become a major ornamental potted plant, and is an important component of the U.S. floral industry. Susceptibility to insect pests and diseases caused by pathogens remains a problem for poinsettia production, even under greenhouse conditions. While chemical treatment can control certain insect pests and disease pathogens, such treatment can also have an adverse effect upon poinsettias. The methods described herein provide a means to genetically engineer transgenic poinsettia that express macromolecules capable of protecting the plant against the insects and pathogens. The production of transgenic plants can also be used to enhance the commercial value of poinsettia by controlling characteristics such as flower color.

Production of transgenic poinsettia

This invention relates to a new and distinct June-bearing cultivar of strawberry plant named 'Clancy' primarily adapted to the growing conditions of west central New York and other regions of similar climate. The new cultivar is primarily characterized by strong vigor, large flowers, broader than long fruit, globose fruit shape, very large primary fruit, dark red fruit color, very firm fruit flesh, the upright attitude of pedicels and fruiting trusses, and late season fruit maturation.

Strawberry plant named 'Clancy'

This invention relates to a new and distinct June-bearing cultivar of strawberry plant named 'L'Amour' primarily adapted to the growing conditions of west central New York and other regions of similar climate. The new cultivar is primarily characterized by strong vigor, large flowers, longer than broad fruit, conical fruit shape, uniformity in shape between primary and secondary fruit, bright red fruit color, glossy skin, very firm fruit flesh, large reflexed calyx, and long pedicels and fruiting trusses.

Strawberry plant named 'L'Amour'

The invention provides a method for producing stably transformed dicotyledonous plants, via the introduction of a foreign DNA, containing an expression vector carrying a gene of interest, into dicotyledonous cells via one or more microparticle bombardments to produce transformed dicotyledonous cells; followed by the regeneration of a stably transformed fertile dicotyledonous plant from the transformed dicotyledonous cells.

Stable transformation of plant cells

A method for conferring resistance to a parasite to a host of the parasite, which comprises isolating a gene fragment from the parasite and inserting the gene fragment or a DNA or RNA segment substantially homologous to the gene fragment or to a DNA or RNA sequence functionally equivalent to the gene fragment into the host, wherein (1) transcription of the gene fragment or the DNA or RNA segment in the host occurs in an anti-sense direction, (2) the gene fragment or the DNA or RNA segment is expressed as a gene product in the host, wherein the gene product is capable of disrupting an essential activity of the parasite, or (3) the gene fragment or the DNA or RNA segment is a binding site capable of competing with a native binding site in the parasite, is disclosed along with hosts produced by this process. Particularly preferred is conferring resistance using a gene fragment from a replicase gene of an RNA virus.

Parasite-derived resistance

Fertile, stably transformed plants regenerated from culture, produced by one or more bombardments of target cells or tissues with microprojectiles coated with foreign DNA comprising one or more genes coding for a desired trait, and selection of transformed cells in culture, are provided.

The invention provides antimicrobial peptides, nucleic acid constructs encoding them, methods for transforming plant cells, and transgenic plant tissue that expresses the antimicrobial peptide genes and thereby exhibit improved resistance to plant pathogens.

Expression of magainin and PGL classes of antimicrobial peptide genes in plants, and their use in creating resistance to multiple plant pathogens

A method of transferring a gene to vertebrate cells is disclosed. The method comprises the steps of: (a) providing microprojectiles, the microprojectiles carrying polynucleic acid sequences, the sequences comprising, in the 5&prime; to 3&prime; direction, a regulatory sequence operable in the tissue cells and a gene positioned downstream of the regulatory sequence and under the transcriptional control thereof; and (b) accelerating the microprojectiles at the cells, with the microprojectiles contacting the cells at a speed sufficient to penetrate the cells and deposit the polynucleic acid sequences therein. Preferably, the target cells reside in situ in the animal subject when they are transformed. Preferred target cells are dermis or hypodermis cells, and preferred genes for insertion into the target cells are genes which code for proteins or peptides which produce a physiological response in the animal subject.

Particle-mediated bombardment of DNA sequences into tissue to induce an immune response

A new and distinct cultivar of red raspberry plant named 'Encore' matures a high percentage of fruit from late July to early August in the East Coast and Great Lakes regions, thereby extending the summer harvest season and decreasing the gap between the summer and fall harvest seasons.

Red raspberry plant named 'Encore'

A new and distinct cultivar of red raspberry plant named 'Prelude' is a very early maturing summer red raspberry cultivar available for production in the East Coast and Great Lakes regions, peaking in production in late June and very early July.

Red raspberry plant named 'Prelude'

A biolistic apparatus is provided with a housing having a carrier membrane disposed in a chamber for movement relative to an outlet plate having a plurality of apertures therein. The carrier membrane has a plurality of micro-particles adhered thereto on the surface of the membrane adjacent the apertures for delivery into cells or tissue. A plurality of rupturable membranes are mounted in spaced relation to the carrier membrane. Initially, a vacuum is provided within the assembly including the outlet opening to purge the apparatus of any undesirable gasses. High pressure helium is then delivered to the rupturable membranes and upon reaching a predetermined pressure, will cause the membranes to rupture allowing a shock front of high pressure helium to strike the carrier membrane and impel the articles through the outlet openings.

Biolistic apparatus for delivering substances into cells and tissues

The invention provides methods for producing a fertile, stably transformed, Zea mays plant. The methods comprise the steps of (a) providing a foreign DNA comprising an expression vector carrying a gene encoding an agronomic trait; (b) providing a maize embryogenic callus, suspension culture, or immature embryo isolated from a plant; (c) introducing the foreign DNA into the embryogenic callus, suspension culture or immature embryo isolated from a plant by one or more microparticle bombardments; and (d) regenerating fertile transgenic Zea mays plant.

Fertile, stably transformed Zea mays plants can be produced by introducing agronomic trait-encoding DNA, via microprojectile bombardment, into maize embryonic callus or suspension culture that has been broken into clumps having a maximum diameter of about 650 microns, where the bombardment is effected within six days of the formation of the clumps.

Growing community of inventors

Geneva, NY, United States of America

John C Sanford