Cognis Corporation

Other

Cognis IP Management Gmbh

John C Loper

Krishna M Madduri

David L Craft

Maria Tang

C Ron Wilson

L Dudley Eirich

Mark Eshoo

Cathy A Cornett

Alfred A Brenner

Martin Anthony Gleeson

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the &#x3c9;-hydroxylase complex of20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

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A novel gene has been isolated which encodes cytochrome b5 (CYTb5) protein of the &ohgr;-hydroxylase complex of 20336. Vectors including this gene, and transformed host cells are provided. Methods of increasing the production of a CYTb5 protein are also provided which involve transforming a host cell with a gene encoding this protein and culturing the cells. Methods of increasing the production of a dicarboxylic acid are also provided which involve increasing in the host cell the number of genes encoding this protein.

Cytochrome b5 gene and protein of Candida tropicalis and methods relating thereto

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the &ohgr;-hydroxylase complex of,20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

Cytochrome P450 monooxygenase and NADPH cytochrome P450 oxidoreductase genes and proteins related to the omega hydroxylase complex of Candida tropicalis and methods relating thereto

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Cincinnati, OH, United States of America

John C Loper