Arizona State University

University of California

John C Chaput

Matthew Dunn

Hao Yan

Andrew Larsen

Jen-Yu Liao

Saikat Bala

Stuart Lindsay

Stephen Albert Johnston

Joshua LaBaer

Peiming Zhang

Neal W T Woodbury

Bertram L Jacobs

Ji Qiu

Christopher W Diehnelt

Mitch Magee

Justin Saul

Sujay Sau

Brian Wellensiek

Julia Flores

The invention provides compositions and methods for synthesis of phosphorylated organic compounds, including nucleoside triphosphates.

Compositions and methods for synthesis of phosphorylated molecules

The invention provides xeno-nucleic acid particle display libraries, methods for identifying functional non-natural nucleic acid (XNA) aptamers using the particle display libraries, and compositions comprising XNA aptamers identified by screening candidate molecules using the xeno-nucleic acid particle display libraries.

Screening artificial nucleic acids by particle display

The present invention is directed to a polymerase activity assay that produces a strong optical signal when a primer-template complex is extended to full-length product. The assay uses Cy3 as the molecular beacon and Iowa Black&#xae; RQ as the quencher. The signal-to-noise-ratio (STNR) of this donor-quencher pairing is &#x2dc;200-fold over background, which is considerably better than other donor-quencher pairs (STNRs &#x2dc;10-20-fold). The STNR allows for solution-based monitoring of polymerase activity. Because the sensor functions via Watson-Crick base pairing, the polymerase activity assay may also be used to evolve polymerases to accept xeno nucleic acids as substrates.

Highly sensitive optical sensor for polymerase screening

Methods for synthetic antibodies, methods for making synthetic antibodies, methods for identifying ligands, and related methods and reagents.

Synthetic antibodies

Methods and compositions for replication of threose nucleic acids (TNAs) are described. The described methods include a method for transcribing a DNA template into a TNA, and a method for reverse transcribing a threose nucleic acid into a cDNA.

Modified polymerases for replication of threose nucleic acids

The present invention provides stable, nuclease-resistant TNA and TNA-DNA oligonucleotides, wherein the oligonucleotides are completely resistant to enzymatic degradation for at least 24-72 hours. Methods of synthesis and use in diagnostic and therapeutic applications are also provided. Specifically, in one embodiment, we describe the chemical and biological stability of TNA and mixed-backbone (mosaic) TNA-DNA oligonucleotides under a variety of conditions and sequence contexts.

Nuclease-resistant DNA analogues

A method and related microfluidic chip and kit for high throughput detection of proteins of interest contained in a sample is disclosed. The method comprises of specifically labeling fusion proteins in a complex sample with fusion tag specific fluorophores that specifically bind the fusion tags coupled to the proteins of interest, and subjecting the sample to automated capillary electrophoresis, wherein the presence of the proteins of interest in the sample is detected by fluorescence signals associated with the fusion tag specific fluorophores.

High throughput detection of fusion proteins

The present invention provides isolated polynucleotides that can serve as translation enhancing elements and their use in protein expression reagents and methods.

Genetic element that enhances protein translation

The present invention provides finite, addressable, and self-assembling nucleic acid tiling arrays, and methods for their use.

Growing community of inventors

Phoenix, AZ, United States of America

John C Chaput