New England Biolabs, Inc.

John Buswell

Christopher H Taron

Ming-Qun Xu

Ivan R Correa, Jr

Laurence Ettwiller

Ellen P Guthrie

Ira Schildkraut

George Tzertzinis

Madalee G Wulf

Paula Magnelli

Erbay Yigit

A method of labeling, and optionally enriching, for a population of target RNA molecules in a mixture of RNAs is provided. In some embodiments, the method may comprise (a) adding a label to the 5' end of 5&#x2032;-diphosphorylated or 5&#x2032;-triphosphorylated target RNA molecules in a sample by incubating the sample with labeled GTP and a capping enzyme; and (b) optionally enriching for target RNA comprising the affinity tag-labeled GMP using an affinity matrix that binds to the affinity tag. The label may be an oligonucleotide, which may further comprise an affinity group attached either internally or at 5&#x2032; or 3&#x2032; end of the oligonucleotide where the oligonucleotide label may be added directly, or indirectly via a reaction with a reactive group to the target RNA.

Methods for labeling a population of RNA molecules

Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.

Deglycosylation reagents and methods

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Byfield, MA, United States of America

John Buswell