Other

Cell Signaling Technology, Inc.

John B Rush

Michael Comb

Hui Zhang

Yi Tan

Xiangming Zha

Dustin J Rush

A physiologic test, and a system for implementing this test, including, from a methodologic point of view, (a) illuminating the central field in a test subject's eye with a relative-motion test image produced on an image display structure by a motion-image-creation structure, (b) by such illuminating, creating a related, subject-perception image, (c) requesting a subject report describing the observed presence and nature, if any, of a distortion, relative to the test image, in the perception image, and (d) thereafter utilizing such a report to assess a test subject's physiologic condition involving macular, paramacular, and neural-pathway physiologic degeneration. A preferred test image, which is fully adjustable by a test administrator with respect to substantially all of its image parameters, takes the form of an image field of elongate, spaced, parallel lines, having edges which distinctly contrast with a background field, and which move smoothly and linearly across the image field.

Eye-administered, moving-image physiologic test system and method

The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS&#x2014;MS), and/or MSanalysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

Immunoaffinity isolation of modified peptides from complex mixtures

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Gold Beach, OR, United States of America

John B Rush