Roche Vitamins Inc.

Dsm IP Assets B.v.

Hoffmann-la Roche Inc.

Dsm Nutritional Products Inc.

John B Perkins

Janice G Pero

R Rogers Yocum

Alan Sloma

Thomas John Erdenberger

Theron Hermann

Randolph T Hatch

Stanley Grant Bower

Ghislain Schyns

Rual Lopez-Ulibarri

Markus G Goese

Markus Huembelin

Abel Ferrandez

Michèle Schaber

Scott W Van Arsdell

A method to increase the production of a desired chemical compound in a microorganism by introduction of a DNA sequence at the 5' end of the encoding DNA gene sequence capable of forming a stem loop and capable of increasing the stability of mRNA transcripts from one or more genes, thus stabilized mRNAs, corresponding DNA sequences and microorganisms.

Method for mRNA stabilization

The present invention provides a method for producing thiamin products using a microorganism containing a mutation that causes it to overproduce and release thiamin products into the medium. Biologically pure cultures of the microorganisms and isolated polynucleotides containing the mutations are also provided. In addition, methods for detecting a pathogenic microorganism in a clinical sample, assays for identifying an antibiotic, as well as, antibiotics identified by such assays are provided.

Thiamin production by fermentation

The present Invention relates to enzymes involved in the Synthesis of Coenzyme Q-10, i.e., decaprenyl diphosphate (DPP) synthase and 4-hydroxybenzoate polyprenyltransferase, to isolated DNA encoding said enzymes and to methods for the microbial production of Coenzyme Q-10.

Microbial production of COQ10

Vectors and recombinant bacteria for overproducing riboflavin, in which nucleic acid overproducing riboflavin biosynthetic proteins is introduced in the chromosome of the host organism, e.g. at multiple sites and in multiple copies per site. A rib operon having at least five genes is used to make such recombinant bacteria.

Bacterial strains which overproduce riboflavin and methods of use thereof

The invention generally provides the genes of the biotin biosynthetic operon of, and closely related species thereof, and well as constructions useful for high level production of biotin.

Biotin biosynthesis in bacillus subtilis

A method is disclosed for the increased production of biotin and the biotin precursor dethiobiotin using a bacterium that produces a lysine-utilizing DAPA aminotransferase. This method involves the use of a bacterium that is either grown in the presence of lysine or deregulated for lysine biosynthesis.

Overcoming DAPA aminotransferase bottlenecks in biotin vitamers biosynthesis

Nutrient medium for bacterial strains which overproduce riboflavin

The invention generally provides the genes of the biotin biosynthetic operon of,, and closely related species thereof, and well as constructions useful for high level production of biotin.

The invention generally provides the genes of the biotin biosynthetic operon of Bacillus subtilis, and closely related species thereof, and well as constructions useful for high level production of biotin.

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Reading, MA, United States of America

John B Perkins