Synthego Corporation

John Andrew Walker, Ii

Reed Kelso

Travis Maures

Jared Carlson-Stevermer

Anastasia Kadina

Sahil Joshi

Paul Dabrowski

Fabian Gerlinghaus

The present invention provides improved automated systems and methods for synthesis of biopolymers including DNA and RNA. The automated systems and methods represent a number of improvements over existing systems for multiplex synthesis of biopolymers in a combinatorial fashion.

Guide RNA synthesis system and method

Provided herein are polynucleotides and CRISPR effector proteins configured to be covalently bound together in a CRISPR complex. The polynucleotides can be further modified to modulate the activity of the CRISPR complex. Modification of the polynucleotide and CRISPR effector protein can be used to improve the efficacy of target binding and/or cleavage.

Stabilized CRISPR complexes

Provided herein are polynucleotides comprising sequence configured to bind to a CRISPR effector protein. Modulation of one or more modifications of the polynucleotides can be used to tune the activity of CRISPR effector proteins complexed with the polynucleotides.

Systems and methods for modulating CRISPR activity

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San Leandro, CA, United States of America

John Andrew Walker, Ii