Sionex Corporation

Netbio, Inc.

Ande Corporation

Dh Technologies Development Pte. Ltd.

John A Wright

Raanan A Miller

Erkinjon G Nazarov

Gary Alan Eiceman

Eugene Tan

Gregory John Kellogg

Richard F Selden

Heung Chuan Lam

Douglas B Cameron

David B Wheeler

Ulrich Hans Thomann

C James Morris

Quan Shi

Valery Leonidovich Bogdanov

Evgeny Krylov

Stephen D Kendig

Heidi Susanne Giese

Clayton Morris

Stephen L Coy

Evgeny Krylova

Hans Thomann Ulrich

Heidi S Giese

Heung C Lam

The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.

Integrated nucleic acid analysis

Integrated systems for the multiplexed amplification and detection of six and greater dye labeled fragments

A fast, multiplexed PCR system is described that can rapidly generate amplified nucleic acid products, for example, a full STR profile, from a target nucleic acid. Such systems include, for example, microfluidic biochips and a custom built thermal cycler, which are also described. The resulting STR profiles can satisfy forensic guidelines for signal strength, inter-loci peak height balance, heterozygous peak height ratio, incomplete non-template nucleotide addition, and stutter.

Methods for rapid multiplexed amplification of target nucleic acids

A ion mobility-based analyzer system including a first ion mobility-based filter for passing selected ions through a time-varying field where the time-varying field being compensated by an adjustable compensation setting. The analyzer also includes a second ion mobility-based filter for receiving a first portion of ions from the first ion mobility-based filter. The second ion mobility-based filter includes a voltage gradient for separating ions of the first portion of ions where the ions have associated retention times based on their times of flight through the voltage gradient. The second ion mobility-based filter includes a detector for detecting the ions at their retention times. The analyzer system further includes a display that displays the detected ions in a plot relating the retention times of the ions in the second ion mobility-based filter with compensation settings of the first ion mobility-based filter.

Method and apparatus for enhanced ion mobility based sample analysis using various analyzer configurations

The invention relates generally to ion mobility based systems, methods and devices for analyzing samples and, more particularly, in some embodiments to compact GC-DMS systems, methods, and devices employing enhanced GC column temperature control and configurations to identify various sample constituents.

Explosives detection using differential mobility spectrometry

The invention provides, in various embodiments, systems and methods relating to controlling ion behavior in an ion-based analysis system.

Systems and methods for ion mobility control

An ultra compact ion mobility based analyzer in a multilayered chip assembly employing various features such as a ion flow generator to propel ions through an ion mobility based filter and, thereby, reduce analyzer size, cost, and power requirements.

Ultra compact ion mobility based analyzer apparatus, method, and system

The invention relates generally to ion mobility based systems, methods and devices for analyzing samples and, more particularly, in some embodiments to compact GC-DMS systems and methods, and techniques for correcting nonlinear characteristics in an ion mobility based analyzer.

Compact sample analysis systems and related methods of using combined chromatography and mobility spectrometry techniques

The invention, in various embodiments, is directed to an analyzer using a solid-state flow generator to provide effluent flow along a flow path and deliver a sample to an ion mobility based filter and detector for analysis.

Solid-state flow generator and related systems, applications, and methods

A system for analyzing one or more ion species of a sample including a first ion mobility filter associated with a first flow path for passing first ions of the sample, a second ion mobility filter associated with a second flow path for passing second ions of the sample, a first outlet from the first flow path for passing a portion of the first ions from the first flow path to the second flow path, and a first outlet from the second flow path for removing neutral particles from the second flow path where the first outlet from the second flow path is upstream of the second ion mobility filter in relation to the ion flow in the second flow path.

An RF-driven plasma source, including a pair of spaced-apart plasma electrodes, wherein the electrodes act as plates of a capacitor, the gas electrically discharges and creates a plasma of both positive and negative ions, in a clean process that enables efficient sample analysis, with preferred isolated sample photo-ionization, reduced-power operation and also including signal detection with modulated drive electronics.

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Billerica, MA, United States of America

John A Wright