Other

Dade International Inc.

John A Todd

John A Kink

Michael E Jolley

Dinesh O Shah

Terukatsu Arima

Terence E Ryan

Badr Saeed

David C Leahy

Delia R Bethell

An assay for detection of serum antibodies having specificity to hepatitis C viral antigens utilizes the carboxy-terminal 102 amino acids of the c33c region of nonstructural gene 3 of HCV containing substantially all the epitopes of the gene 3 protein. In the preferred embodiment the recombinant 102 amino acid peptide is coated onto a solid support, and incubated in the presence of serum obtained from a patient infected with HCV. The presence of antibodies bound to the antigen coated support is detected in a sandwich format utilizing a second reporter-conjugated antibody.

Epitope mapping of the c33 region of HCV

New assays for diagnosing NANBH utilizing novel peptide fragments derived from polypeptide antigens reactive to antibodies present in the sera of infected patients are disclosed. In producing these peptides, the portion of the polypeptide contributing to high backgrounds is deleted thereby resulting in assays with an exceptionally high signal to background ratio.

Assay for Non-A Non-B hepatitis

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Grayslake, IL, United States of America

John A Todd