Restoration Robotics, Inc.

Berkeley Lights, Inc.

Other

Bruker Cellular Analysis, Inc.

Venus Concept Ltd.

Bruker Cellular Analysic, Inc.

John A Tenney

Troy A Lionberger

Hui Zhang

Shehrzad Ahmed Qureshi

Kyle R Breton

Paul M Lundquist

Mohan Bodduluri

Fenglei Du

Hansohl E Kim

Thomas M Vetterli

Eric D Hobbs

Brandon R Bruhn

Robert F Biro

Keith J Breinlinger

J Tanner Nevill

Clifford A Oostman, Jr

Gabriele Zingaretti

Jian Gong

Aathavan Karunakaran

Randall D Lowe, Jr

Stephen B Leighton

I-Jong Lin

Vincent Haw Tien Pai

Erik R Burd

Shehrzad A Qureshi

Gang F Wang

Gregory G Lavieu

Christopher C Shing

Angel Navas Angeles

Raziel Solomon Alon

Peter J Beemiller

Natalie C Marks

Guido K Stadler

Yelena Bronevetsky

(Radhika) Mohan Bodduluri

Joshua Foster Slocum

Hayley M Bennett

Darshan Thaker

Matthew E Fowler

Kevin D Loutherback

Jason M Mcewen

Yogesh Khemchandra Dhande

Patrick N Ingram

Erin Chia-wei Hsi

John Junyeon Kim

Annamaria Mocciaro

Amanda L Goodsell

Duane Smith

Elisabeth Marie Walczak

Phillip J M Elms

Anupam Singhal

Christoph Andreas Neyer

Alona Kostenko

Alexander Gerald Olson

Wesley Arthur Zink

Oleksandr Tokmakov

Igor Fastnacht

Yuriy Nicheporuk

Andriy Koval

Khrystyna Andres

Joshua F Slocum

Jr Randall D Lowe

Systems for operating a microfluidic device are described. The systems comprise a first surface configured to interface and operatively couple with a microfluidic device and a lid configured to retain the microfluidic device on the first surface. The lid comprises a first portion having a first fluid port configured to operatively couple with and flow fluidic medium into and/or out of a first fluid inlet/outlet of the microfluidic device and a second portion having a second fluid port configured to operatively couple with and flow fluidic medium into and/or out of a second fluid inlet/outlet of the microfluidic device. The second portion of the lid is separable from the first portion and movable between a closed position in which the second fluid port of the second portion of the cover is operatively coupled with the second fluid inlet/outlet of the microfluidic device and an open position in which a portion of the microfluidic device that contains the second fluid inlet/outlet is exposed. Other embodiments are described.

Systems for operating microfluidic devices

Methods are provided for the assay of secreted biomolecules using automated detection and characterization of micro-objects in a microfluidic device. The biomolecules can be secreted by cells, particularly immunological cells, such as T cells. The biomolecules being assayed can include cytokines, growth factors, and the like. Methods are also provided for assaying the cytotoxicity of a cell with respect to another, target cell. Also provided are kits and non-transitory computer-readable media in which programs are stored for causing a system comprising a computer to perform automated methods for detecting secreted biomolecules and/or cytotoxicity in a microfluidic device.

Methods for assaying biological cells in a microfluidic device

Methods are provided for the automated detection of micro-objects in a microfluidic device. In addition, methods are provided for repositioning micro-objects in a microfluidic device. In addition, methods are provided for separating micro-objects in a spatial region of the microfluidic device.

Automated detection and repositioning of micro-objects in microfluidic devices

Methods are provided for the automated detection, characterization, and selection of micro-objects in a microfluidic device. In addition, methods are provided for grouping detected micro-objects into subgroups that share the same characteristics and, optionally, repositioning micro-objects in a selected sub-population within the microfluidic device. For example, micro-objects in a selected sub-population can be moved into sequestration pens. The methods also provide for visual displays of the micro-object characteristics, such as two- or three-dimensional graphs, and for user-based definition and/or selection of sub-populations of the detected micro-objects. In addition, non-transitory computer-readable medium in which a program is stored and systems for carrying out any of the disclosed methods are provided.

Automated detection and characterization of micro-objects in microfluidic devices

Disclosed are methods, systems, and articles of manufacture for performing a process on biological samples. An analysis of biological samples in multiple regions of interest in a microfluidic device and a timeline correlated with the analysis may be identified. One or more region-of-interest types for the multiple regions of interest may be determined; and multiple characteristics may be determined for the biological samples based at least in part upon the one or more region-of-interest types. Associated data that respectively correspond to the multiple regions of interest in a user interface for at least a portion of the biological samples in the user interface based at least in part upon the multiple identifiers and the timeline. A count of the biological samples in a region of interest may be determined based at least in part upon a class or type of data using a convolutional neural network (CNN).

Systems and methods for analyses of biological samples

Methods and systems are provided useful in various procedures, including hair harvesting and implantation, and further including computer-implemented and/or robotic hair transplantation. Methodologies are provided which enable a tool, such as a hair harvesting or a hair implantation tool, to proceed at least under a partial computer control in a selected direction of travel along a donor or recipient area of the patient, as well as changing direction of travel based on desired harvesting and/or implantation criteria.

Methods and systems for directing movement of a tool in hair transplantation procedures

Optically-actuated microfluidic devices permit the use of spatially-modulated light to manipulate micro-objects such as biological cells. Systems and methods are described for providing sequences of light patterns to move and direct a plurality of micro-objects within the environment of a microfluidic device. The sequenced light patterns provide improved efficiency in directing the transport of the plurality of micro-objects. Other embodiments are described.

Light sequencing and patterns for dielectrophoretic transport

Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.

Methods, systems and kits for in-pen assays

Methods are provided for the automated detection and/or counting of micro-objects in a microfluidic device. In addition, methods are provided for repositioning micro-objects in a microfluidic device. In addition, methods are provided for separating micro-objects in a spatial region of the microfluidic device.

Systems and methods for tracking, identifying, labeling and/or classifying objects or features of interest, such as follicular units are provided. In some embodiments the identified and labeled features of interest, such as follicular units, may be used for planning hair harvesting, hair implantation and/or performing hair transplantation procedures.

Object-Tracking systems and methods

A system and method for tracking, identifying, and labeling objects or features of interest, such as follicular units is provided. In some embodiments, tracking is accomplished using unique signature of the follicular unit and image stabilization techniques. According to some aspects pixel data of a region of interest in a first image is compared to pixel data of the regions of interest in a second image, and based on a result of the comparison of pixel data in the region of interest in the first and second images and the signature of the follicular unit, locating the follicular unit in the second image. In some embodiments the follicular unit is searched for in the direction of a motion vector.

Object-tracking systems and methods

Growing community of inventors

Piedmont, CA, United States of America

John A Tenney