Her Majesty the Queen in Right of Canada, as Represented by the Minister of Agriculture and Agri-Food

Her Majesty the Queen in Right of Canada as Represented by Agriculture

Her Majesty in Right of Canada, as Represented by the Minister of

Agri-Food Canada

John A Simmonds

Linda J Harris

Leslie Cass

Sharon Allard

Laurian Sylvio Robert

Brian Miki

Teresa Martin-Heller

Keqiang Wu

Stephen Gleddie

Kamal Malik

Jas Singh

Th&eacute;r&egrave;se Ouellet

Jean L Gerster

Dan Brown

Titus Tao

Ming Hu

This invention is directed to a regulatory region obtained from a wheat aleurone gene LtpW1. This regulatory region, truncated derivatives, mutations, or deletions of this regulatory region, can be used to express heterologous genes of interest within aleurone cells of a plant. Furthermore, this invention is directed to a truncated LtpW1 regulatory region that exhibits constitutive activity with both monocot and dicot plants. This invention is also directed to vectors comprising these regulatory regions operatively linked with a heterologous gene of interest, as well as plant cell cultures and transgenic plants comprising these vectors. A method for the preparation of a plant using the regulatory regions of this invention are also disclosed.

Regulatory region of a lipid transfer protein (LtpW1) from aleuron tissue of wheat

is a plant pathogen, attacking a wide range of plant species including corn (ear and stalk rot), barley, and wheat (head blight).epidemics result in millions of dollars of losses in crop revenues.infection in the cereals reduces both grain yield and quality. Mycotoxins are produced by many fungalspecies and thus the grain becomes contaminated with these mycotoxins, such as the trichothecenes. The major trichothecene produced byis deoxynivalenol (abbreviated as DON, also known as vomitoxin). Trichothecenes are potent protein synthesis inhibitors and are quite toxic to humans and livestock. A yeast gene has been identified which confers upon yeast tolerant of the trichothecene, trichodermin. A corresponding plant gene has been prepared, which has been used to transform plants. These transformed plants have an increased resistance toinfestation.

Tolerance of trichothecene mycotoxins in plants through the modification of the ribosomal protein L3 gene

The invention can be summarized as follows. Several silk-specific genes were isolated from corn silk. The specificity of their expression was determined by Northern analysis. A regulatory region was obtained from the silk gene, C3, and found to direct silk, and pistil, expression in transient assays and transgenic plants, in both monocot and dicot plants. The regulatory region of a silk gene may be used to drive the expression of a gene of interest within pistil or silk-tissues for a range of utilities including pathogen resistance or female sterility.

Corn silk gene and regulatory region

This invention is directed to a regulatory region obtained from a wheat aleurone gene LtpW1. This regulatory region, or truncated derivatives of this regulatory region, can be used to express heterologous genes of interest within aleurone cells of a plant. Furthermore, this invention is directed to a truncated LtpW1 regulatory region that exhibits constructive activity within both monocot and dicot plants. This invention is also directed to vectors comprising these regulatory regions operatively linked with a heterologous gene of interest, as well as plant cell cultures and transgenic plants comprising these vectors. A method for the preparation of a plant using the regulatory regions of this invention are also disclosed.

Wheat aleurone regulatory region

This invention is directed to regulatory elements obtained from a wheat aleurone gene LtpW1. The regulatory element, analogs thereof, or truncated derivatives of this regulatory element, can be used to express heterologous genes of interest within the aleurone cells of a plant. This invention is also directed to vectors comprising these regulatory elements operatively linked with a heterologous gene of interest, as well as plant cell cultures and transgenic plants comprising these vectors. A method for the preparation of a plant using the regulatory elements of this invention are also disclosed. Furthermore, this invention is directed to a truncated LtpW1 regulatory element that exhibits constitutive activity within plants.

Wheat aleurone regulatory elements

A Brassica sp. genomic clone G 363 containing a gene promoter, which directs expression in the stigma, was isolated. Based on microbombardment studies of Brassica napus flowers with the gene Pis 63/Gus fusion, the Pis 63 promoter was shown to direct GUS expression to the stigma. This promoter will be useful for the temporal and spatial control of gene expression in plants.

Brassica sp. gene promoter highly expressed during stigma development

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Ottawa, Canada

John A Simmonds