Corning Incorporated

John A Ryan

William Joseph Lacey

Kathy Marie Youngbear

Gregory Mathus

David M Root

Joseph Christopher Wall

Todd M Upton

Laurence M Vaughan

The present invention relates generally to a cell culture stirring vessel and associated impeller. More particularly, it relates to a fully integrated, disposable spinner flask vessel having a suspended impeller assembly permanently integrated therein. The invention is particularly suitable for use in applications where cells are suspended within a liquid medium with minimal shear forces.

Disposable spinner flask

A stackable flask for the culturing of cells is disclosed. The cell culture chamber is defined by a top plate and a rigid bottom tray of substantially rectangular shape connected by side and end walls, the body of the flask has imparted therein a gas permeable membrane that will allow the free flow of gases between the cell culture chamber and the external environment. The flask body also includes a sealed septum that will allow access to the cell growth chamber by means of a needle or cannula. The size of the flask and location of an optional neck and cap section allows for flask manipulation by standard automated assay equipment, making the flask ideal for high throughput applications.

Cell cultivating flask

A high-throughput cell or tissue culture apparatus, which is configurable to an industry-standard well plate format, is provided. The apparatus comprises a number of vessels, which may be suspended in wells of a plate. Each vessel has at least one sidewall defining a first opening and a second opening, each of predetermined cross-sectional area. The second opening has an inner cross-sectional area greater than either the inner cross-sectional area of the first opening or a cross-sectional area in a horizontal plane between the first and second openings. A relatively large substrate area is provided in each vessel for supporting tissue cultures in a fluid medium.

Multi-well assembly for growing cultures in-vitro

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Clinton, MA, United States of America

John A Ryan