Illumina Corporation

Cidra Corporation

Cyvera Corporation

Illumina Cambridge Limited

Spectra Science Corporation

Other

Spectra Systems Corporation

Electronics and Telecommunications Research Institute

Moon, John A.

John A Moon

Martin A Putnam

Alan D Kersey

Tuo Li

Stephen Tanner

Joseph Francis Pinto

Paul S Szczepanek

James R Dunphy

Francisco Jose Garcia

Tobias Mann

Klaus Maisinger

Anthony James Cox

Michael Lawrence Parkinson

Haifang H Ge

M Shane Bowen

David Fournier

Hongji Ren

Nabil M Lawandy

Stanley S Hong

Michael A Davis

James S Sirkis

Dale Buermann

Maria Candelaria Rogert Bacigalupo

Mark Wang

Darren Segale

Matthew William Hage

Mark J Nibbe

Bryan Crane

Jason Harris

Joseph Traynor

Kevin L Gunderson

Jonathan Mark Boutell

Yir-Shyuan Wu

Jay Walter Dawson

Jeffrey S Fisher

Shengrong Lin

Bala Murali Venkatesan

Martin Maria Fabani

Gary Mark Skinner

Alex Nemiroski

Geraint Wyn Evans

Steven Modiano

Michel Perbost

Christopher Chestnut

Jason Richard Betley

Ryan Christopher Smith

Michael S Graige

Steven H Modiano

Anthony Rauseo

Helmy A Eltoukhy

Robert C Kain

Min-Jui Richard Shen

Ryan M Smith

Robert N Brucato

Michal Lebl

Christian Victor O'Keefe

Charles Winston

John S Vieceli

Ralph W Jones

Kenny Chen

Boyan Boyanov

James C Dunphy

Steven Mark Barnard

Jeffrey G Mandell

Merek C Siu

Shaoping Lu

David R Fournier

F Kevin Didden

Shane Bowen

James Sirkis

Ralph Jones

In one aspect, the disclosed technology relates to systems and methods for sequencing polynucleotides. In one embodiment, the disclosed technology relates to a nanopore sensor device for identifying nucleotides, the nanopore sensor device including: one or more cis wells; one or more cis electrodes associated with the one or more cis wells; a plurality of trans wells, each of the plurality of trans wells separated from the one or more cis wells by a lipid or solid-state membrane having a nanopore; a plurality of field effect transistors (FETs), each of the plurality of FETs associated with one of the plurality of trans wells; an electrical source configured to provide alternating current (AC) inputs between the one or more cis electrodes and the source terminals of the plurality of FETs; and a controller operably coupled to the plurality of FETs, the controller configured to measure AC responses of the plurality of FETs, wherein the AC responses depend on the identities of the nucleotides within or near the nanopores.

Scalable circuit for molecular detection

A structured illumination microscopy (SIM) system comprises: a light source; a light-structuring component to provide light from the light source with a SIM pattern for performing illuminations of a sample at a substrate having a substrate pattern, wherein a pitch of the SIM pattern is based on a characteristic of the substrate pattern; and an image sensor to detect emissions that the sample generates in response to the illuminations.

Performing structured illumination microscopy on a patterned substrate

Methods and systems for analysis of image data generated from various reference points. Particularly, the methods and systems provided are useful for real time analysis of image and sequence data generated during DNA sequencing methodologies.

Creating a template of nucleic acid site locations on a flow cell

A method is used to generate an analysis image of a moving sample based on one or more exposures. An illumination source illuminates a field of view of a camera for one or more pulses while the sample moves through the field of view. The distance moved by the sample during each of these one or more pulses may be less than the size of one pixel in an image captured by the camera.

Apparatus and method of obtaining an image of a sample in motion

Analyzing image data from nucleic acid sequencing

Identifying nucleotides by determining phasing

Provided is a surface having metal regions and an interstitial region having a composition that differs from the metal regions, wherein a continuous gel layer coats the surface across the metal regions and the interstitial regions. Nucleic acids or other analytes can be attached to the continuous gel layer such that a greater amount is attached over the metal regions than over the interstitial region. Also provided are methods for making such surfaces. Methods are also provided for making an array of nucleic acids or other analytes using such surfaces.

Patterned flow-cells useful for nucleic acid analysis

A method for scanning a microarray, including (a) providing a microarray to an optical scanner, wherein the microarray includes a surface having features having different target molecules; (b) scanning the surface in the x or y direction to acquire optical signals from the features at sequential regions of the surface, wherein the focus setting between the optical scanner and the surface is dynamically controlled in real time by repeatedly (i) adding a predetermined focal offset, thereby introducing an error in the focus setting, and (ii) adjusting the focus setting to reduce the error in the focus setting, thereby acquiring the optical signals from different regions at different degrees of focus such that a subset of the regions that are scanned have an introduced focus error; and (c) analyzing the optical signals that are obtained at the different degrees of focus to identify the different target molecules.

Dynamic autofocus method and system for assay imager

In an example of a method of making a flowcell, an organic solid support including sidewalls and a top is provided. A bottom surface of the organic solid support adjacent to the sidewalls provides a laser bonding foot. In the method, the laser bonding foot is bonded to an inorganic solid support to form a channel having sidewalls and a top defined by the organic solid support.

Multilayer fluidic devices and methods for their fabrication

In an example of the method, a functionalized coating layer is applied in depressions of a patterned flow cell substrate. The depressions are separated by interstitial regions. A primer is grafted to the functionalized coating layer to form a grafted functionalized coating layer in the depressions. A hydrogel is applied on at least the grafted functionalized coating layer.

Flow cells with hydrogel coating

A portable detector is disclosed for detecting certain analytes of interest, such as genetic material (e.g., nucleic acids). The detector includes a reading component for the detection of the analytes, and control circuitry for controlling operation of the reading component. Processing circuitry may be included to perform both primary analysis of acquired data, and where desired, secondary analysis. Where desired, some or all of the computationally intensive tasks may be off-loaded to enhance the portability and speed of the device. The device may incorporate various types of interface, technologies for reading and analysis, positioning system interfaces, and so forth. A number of exemplary use cases and methods are also disclosed.

Portable genetic detection and analysis system and method

System and methods for identifying nucleotides

A detection apparatus having a read head including a plurality of microfluorometers positioned to simultaneously acquire a plurality of the wide-field images in a common plane; and (b) a translation stage configured to move the read head along a substrate that is in the common plane. The substrate can be a flow cell that is included in a cartridge, the cartridge also including a housing for (i) a sample reservoir; (ii) a fluidic line between the sample reservoir and the flow cell; (iii) several reagent reservoirs in fluid communication with the flow cell, (iv) at least one valve configured to mediate fluid communication between the reservoirs and the flow cell; and (v) at least one pressure source configured to move liquids from the reservoirs to the flow cell. The detection apparatus and cartridge can be used together or independent of each other.

Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

A labeled nucleotide includes a nucleotide, a linking molecule attached to a phosphate group of the nucleotide, and a redox-active charge tag attached to the linking molecule. The redox-active charge tag is to be oxidized or reduced by an electrically conductive channel when maintained in proximity of a sensing zone of the electrically conductive channel.

Labeled nucleotides

Growing community of inventors

San Diego, CA, United States of America

John A Moon