Applied Biotechnology Institute, Inc.

Pioneer Hi-bred International, Inc.

Prodigene, Inc.

Other

Genencor International, Inc.

Bgp Products Operations Gmbh

Prodigeue, Inc.

Mazen Animal Health Inc.

Novartis Ag

Washington State University

Ph&t S.p.a.

John A. Howard, Sr.

David Peterson

Elizabeth Hood

Thomas Czapla

John A Howard

Elizabeth E Hood

Marc C Albertsen

Gary A Huffman

Larry R Beach

Celine Hayden

James C Register, Iii

Derrick Witcher

Terry EuClaire Meyer

Thomas H Czapla

Chris L Baszczynski

Sheila E Maddock

Joseph M Jilka

Erin Egelkrout

Gina Fake

Erin Engelkrout

Michael C Ward

David J Peterson

Adrian Edward Smith

Huaming Wang

Wesley B Bruce

David A Peterson

Derrick Ryan Witcher

John D Burr

A Gururaj Rao

Stephen J Streatfield

Fabrizio Niccolai

John Neill

Franciscus J C Van Gastel

Rafael Jimenez-Flores

Jeff R Wood

Michele Bailey Piatchek

Loverine Taylor

Anthony Cavalieri

Gururaj Rao

Susan Woodard

Michele Bailey

James R Wild

T Scott Pinkerton

Kameshwari Pappu

Richard Clough

Stephen Streatfiled

Donna Delaney

Marc C Albertson

Chris Baszycynski

Terry Euclaire Meyer

Iii Register James C

A plant produced vaccine for Porcine Epidemic Diarrhea Virus (PEDV) is provided where the Spike protein of the virus is expressed in a plant by introducing into a plant a construct comprising a promoter preferentially directing expression to seed of said plant, a nucleoic acid encoding the Spike protein and a nucleic acid targeting expression to the endoplasmic reticulum of the plant. The plant expresses the S1 polypeptide at levels of at least 10 mg/kg of seed of said plant. When orally administered to an animal, a protective response is observed including a serum antibody response.

Expression of PEDV sequences in plants and plant produced vaccine for same

Vaccines, methods of producing, and methods of using are provided in which a protective response to Valley Fever disease is produced when administered to an animal. The vaccine provides for expression ofsp. Ag2 polypeptide in a plant or plant part, linked to a promoter preferentially directing expression to seed tissue of the plant or plant part. Further embodiments provide the polypeptide is targeted to the cell wall, vacuole or endoplasmic reticulum. The polypeptide may be fused to a dendritic cell targeting dendritic cell or a heat labile enterotoxin. Increased expression levels in the plant or plant part are obtained. The vaccine comprising the plant-produced Ag2 polypeptide may be a glucan chitin particle comprising the Ag2 polypeptide. The plant or plant materials in an embodiment may be orally administered.

Plant-produced vaccine comprising an AG2 polypeptide from coccidioides

Methods of vaccination of animals are provided. In an embodiment, a paired administration of a primer vaccine provides for non-oral administration of a vaccine and an oral administration of the vaccine and can be followed by a paired administration of a booster vaccine of a non-oral administration and an oral administration. Embodiments provide the non-oral and oral administration are within three days of each other. The methods provide for improved protective response in an animal compared to administration of a primer non-oral administered vaccine followed by three booster non-oral administered vaccines. An adjuvant is provided that is a microcrystalline polysaccharide-based adjuvant derived from delta inulin.

Methods and compositions to increase immune response to vaccines

A method of increasing expression of a product encoded by a nucleic acid molecule is provided where in one embodiment multiple plant transcription units comprising a promoter and the nucleic acid molecule are provided. In another embodiment multiple plant transcription units are provided where each promoter is different from the other. In an embodiment the multiple plant transcription units may comprise two, three, four or more plant transcription units. In another embodiment the promoter may be selected from an embryo promoter. Another embodiment provides the promoter may be a globulin promoter. A further embodiment provides the product encoded may be selected from hepatitis b, aprotinin, or a cellulase. Still further embodiments provide the product may be selected from the cellulases E1 and CBH1.

Method of increasing expression of nucleic acid molecules in plants using multiple transcription units

Increased antigenicity of a membrane-bound polypeptide produced from a plant is provided in a process in which extraction of the polypeptide or other compounds from the plant is such that phospholipids are associated with the polypeptide. Reducing fat by supercritical fluid extraction increases antigenicity of such plant-produced membrane-bound polypeptides. Methods and means of producing such membrane-bound polypeptides are provided. Methods to produce a protective response in animals are provided by administering to the animal the membrane-bound polypeptide. Binding of antibody specific to the membrane-bound polypeptide is increased. The process provides for increased preferred formation of the membrane-bound polypeptide. Stability of the membrane-bound polypeptide is increased when the plant material is defatted.

Methods to increase antigenicity of membrane-bound polypeptides produced in plants

An aerosolization apparatus comprises a body having an inlet, an endpiece having an outlet, the endpiece being connectable to the body to define a chamber, wherein the chamber is sized to receive a capsule containing a pharmaceutical formulation in a manner which allows the capsule to move within the chamber. The apparatus further includes a connection mechanism to provide selective connection of the endpiece to the body, wherein a rotational force between the endpiece and the body is needed to connect or disconnect the endpiece from the body, the rotational force being applied about an axis passing through the chamber. When a user inhales, air enters into the chamber through the inlet so that the pharmaceutical formulation is aerosolized within the chamber and the aerosolized pharmaceutical formulation is delivered to the user through the outlet. The connection mechanism prevents inadvertent disconnection of the endpiece from the body.

Aerosolization apparatus with removable mouthpiece

An enhanced globulin-1 regulatory region is shown, a nucleotide sequence of which includes at least one additional copy of a region of the globulin-1 regulatory region which includes at least one transcriptional factor binding domain, combined with a transcription initiation site and translation start site. The promoter provides improved seed preferred, and particularly embryo preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the embryo tissue of a plant.

Regulatory region having increased expression and method of using same

Aregulatory region is shown, which provides improved seed preferred, and particularly pericarp preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the pericarp tissue of a plant. The sequence is particularly useful in expression of heterologous proteins to the pericarp of monocotyledonous plants, particularly cereals, and maize.

Pericarp tissue preferred regulatory region and method of using same

Saccharification of polysaccharides of plants is provided, where release of fermentable sugars from cellulose is obtained by adding plant tissue composition. Production of glucose is obtained without the need to add additional &#x3b2;-glucosidase. Adding plant tissue composition to a process using a cellulose degrading composition to degrade cellulose results in an increase in the production of fermentable sugars compared to a process in which plant tissue composition is not added. Using plant tissue composition in a process using a cellulose degrading enzyme composition to degrade cellulose results in decrease in the amount of cellulose degrading enzyme composition or exogenously applied cellulase required to produce fermentable sugars.

Methods of saccharification of polysaccharides in plants

Expression of recombinant polysaccharide degrading enzymes in plants is described. In one embodiment, expression of the enzyme is preferentially directed to the seed of the plant. Expression may also be preferentially targeted to specific locations within the plant cell. Expression of cellulases in corn is shown. The result is the capacity to produce polysaccharide degrading enzymes in plants at commercially acceptable levels in a reliable manner. Methods of using same in production of ethanol is also described, including use of the plant-produced enzymes in the ethanol production process.

Monocotyledonous seed expressing exo-1,4B-glucanase

A method of controlling germination of seed is provided in which a nucleic acid encoding a protein which causes inhibition of germination is transformed into a plant, operably linked with a promoter that expresses during germination. The protein is one in which a restoring substance can be applied to the seed and restores germination. Also provided is an amylase germination preferred promoter.

Means and methods of controlling plant seed germination

The invention discloses novel promoter sequences capable of expressing genes in plant cells. The promoters include engineered versions of the maize ubiquitin promoter to increase expression levels beyond those observed with the native ubiquitin promoter and alter the tissue preference. Expression constructs, vectors, transgenic plants and methods are also disclosed.

Plant promoter sequences and methods of use for same

The invention relates to production of proinsulin or insulin in seeds of monocot plants by transformation of monocot plants with a nucleotide sequence encoding proinsulin or insulin under the control of a seed specific promoter.

Commercial production of insulin and insulin-like protein in plants

Increased expression of heterologous proteins in a plant is achieved by introducing the nucleotide sequence encoding the heterologous protein into a plant that has high oil seed content compared to low oil seed and/or is introduced into a plant having reduced alcohol soluble proteins in the endosperm. The nucleotide sequence may be introduced by direct transformation into the plant, or by direct transformation into another plant, and crossing with the high oil plant or plant have reduced levels of alcohol soluble protein in the endosperm. Further, the nucleotide sequences may be introduced into one or both of the high oil plant and the plant having reduced levels of alcohol soluble proteins, and the plants may be crossed to result in a progeny having even further increased expression levels of the heterologous protein.

Method of increasing expression of heterologous proteins in plants

Growing community of inventors

College Station, TX, United States of America

John A Howard