Baxalta Gmbh

Cytokine Pharmasciences, Inc.

John A Baugh

Richard J Bucala

Peter Gregersen

Seamus C Donnelly

Joanita Monteiro

Smita Chitnis

Describe herein is a novel CATT-tetranucleotide repeat polymorphism at position &#x2212;817 of the human Mif that functionally affects the activity of the Macrophage Inhibitory Factor (MIF) promoter in gene reporter assays. Four genotypes are described which comprise 5, 6, 7, or 8-CATT repeat units. Of these, the 5-CATT allele has the lowest level of basal and stimulated MIF promoter activity in vitro. The presence of the low expressing, 5-CATT repeat allele correlated with low disease severity in a cohort of rheumatoid arthritis patients. Methods, compositions and apparatus for detecting this CATT-tetranucleotide repeat polymorphism at position &#x2212;817 of the human Mif gene, and for using same for assessing predisposition to severe inflammatory disease, are also disclosed.

Macrophage migration inhibitory factor (MIF) promoter polymorphism in inflammatory disease

Describe herein is a CATT-tetranucleotide repeat polymorphism at position &#x2212;817 of the human Mif gene that functionally affects the activity of the Macrophage Inhibitory Factor (MIF) promoter in gene reporter assays. Four genotypes are described which comprise 5, 6, 7, or 8-CATT repeat units. Of these, the 5-CATT allele has the lowest level of basal and stimulated MIF promoter activity in vitro. The presence of the low expressing, 5-CATT repeat allele correlated with low disease severity in a cohort of rheumatoid arthritis patients. Methods, compositions and apparatus for detecting this CATT-tetranucleotide repeat polymorphism at position &#x2212;817 of the human Mif gene, and for using same for assessing predisposition to severe inflammatory disease, are also disclosed.

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Kilpedder, Ireland

John A Baugh