Tokyo Medical and Dental University

Fujifilm Corporation

Johji Inazawa

Issei Imoto

Ken-ichi Kozaki

Jun Inoue

Hitoshi Tsuda

Tatsuyuki Kawano

Ryoko Kikuchi

Shinsuke Yamamoto

Hiroyuki Izumi

Dai Ujihara

Junya Yoshida

Sana Yokoi

Takaya Ishihara

Shuhei Komatsu

Hideyuki Kanehara

Erina Nakamura

Wei Yu

Shin Hayashi

Yoshinori Aizu

Ryouko Kikuchi

The present inventors have found microRNAs which are strongly associated with stabilization of NRF2 in tumors, and an object of the present invention is to provide means for utilizing such miRNAs for the diagnosis and treatment of cancer. The inventors conducted screening of 470 microRNAs in a microRNA library by use of HeLa cells. As a result, 8 miRNAs each exhibiting a large decrease in miRNA activity as compared with a control miRNA, and 8 miRNAs each exhibiting a large increase in miRNA activity as compared with a control miRNA, were identified. The inventors have found that the NRF2 activation in the living body, in particular tumor cells, can be detected by use of the thus-identified miRNAs, whereby malignancy of a tumor, or the like can be differentiated. The inventors have also found that a nucleic acid including a miRNA sequence associated with reduction in the aforementioned ARE activity can be used as a cancer therapeutic agent.

Method for assaying MicroRNA, cancer therapeutic agent, and medical composition containing same for cancer therapy

Method for assaying microRNA, cancer therapeutic agent, and medicinal composition containing same for cancer therapy

It is an object of the present invention to identify a gene that exhibits behavior which is characteristic of carcinomas such as thyroid carcinoma, so as to provide a method for detecting carcinoma and a cell growth suppressing agent. The present invention provides a method for detecting carcinoma, which comprises detecting malignant transformation by detecting at least one alteration of gene existing in chromosomal regions 1q41, 3q28, 7q31.2, 8p12, 8q22.2, 8q24.21, 11q4.1, 17q12, 20q11, 9p21.3, 16q13.2, and 16q23.1 in a specimen.

Method for detecting thyroid carcinoma

An object of the present invention is to provide a method for detecting cancer and an agent for suppressing cell growth by identification of genes showing behaviors characteristic in cancer such as esophageal carcinoma. The present invention provides a method for detecting cancer, which comprises detecting canceration through detection of amplification of at least one gene existing in an 1q32-1q41 chromosomal region in a specimen.

Method for detecting esophageal carcinoma and agent for suppressing esophageal carcinoma

An analytical method aided with a nucleic acid microarray, the nucleic acid microarray having a spot (X 1) onto which a first probe nucleic acid is immobilized, the method includes: allowing a labeled sample nucleic acid (A 1) of a sample to be tested to hybridize with the first probe nucleic acid; providing the spot (X 1) with a labeled verification nucleic acid (B) that has a sequence capable of hybridizing with at least a part of the first probe nucleic acid and is labeled with a label different from the labeled sample nucleic acid (A 1), and allowing the labeled verification nucleic acid (B) to hybridize with at least the first probe nucleic acid at all spots; measuring a labeled quantity value (F 1) of the labeled sample nucleic acid (A 1); and measuring a labeled quantity value (Fc 1) of the labeled verification nucleic acid (B).

Method for analysis using nucleic acid microarray

An object of the present invention is to provide a method for detecting cancer through identification of genes exhibiting characteristic behavior in the cases of cancer such as ovarian cancer, and a cell growth inhibitor. The present invention provides a method for detecting cancer, which comprises detecting canceration including malignancy of a specimen through detection of at least one alteration of a gene existing in a chromosomal region 2q14. 2, 3p24. 1, 3q26. 2, 3q29, 4q34. 2, 6q23, 9p21. 3, 11q13. 3, 13q22.1, 13q33. 1, 13q33. 3, 15q12, 15q15. 1, 17p12, 17p13. 1, 17p13. 3, 18q21. 1, 18q21. 2, 18q21. 31, 18q21. 32, 18q21. 33, 18q23, 20q13. 13, 20q13. 2, 20q13. 31, 20q13. 33, Xp11. 23, Xp13.1, Xp13. 3, Xp26. 2, Xp26. 3, or Xq28 in the specimen.

Methods for evaluating the stage of ovarian cancer or the survival rate of an ovarian cancer patient

The present invention is intended to provide a means for judging the malignancy of neuroblastoma and the progress of spontaneous regression thereof. The present invention provides a method for detecting cancer which comprises detecting activation or inactivation of the lysosomal-associated protein multispanning transmembrane 5 (LAPTM5) gene and amplification of the MYCN gene in a specimens to evaluate the malignancy of the specimens and the progress of spontaneous regression.

Method for detecting neuroblastoma

The present invention is directed to methods for suppressing the growth of oral squamous-cell carcinoma cells. The methods include introducing at least one gene selected from miR-137 or miR-193a into oral squamous-cell carcinoma cells.

Method for detecting carcinoma and agent for suppressing carcinoma

It is an object to be achieved by the present invention to provide a method for detecting cancer and a cell growth inhibiting agent by the identification of genes exhibiting characteristic behavior in cancers such as oral squamous-cell carcinoma. The present invention provides a method for detecting cancer, which comprises detecting at least one gene alteration existing in chromosomal region 4q35 in a specimen.

Method for detecting oral squamous-cell carcinoma

An object of the present invention is to provide a method for detecting cancer through identification of genes exhibiting characteristic behavior in the cases of cancer such as anaplastic thyroid cancer. The present invention provides a method for detecting cancer which comprises detecting canceration of a specimen using the amplification of a gene of the p12 region of chromosome 8 in the specimen as an indicator.

Method for detecting cancer and method for suppressing cancer

It is an object of the present invention to provide a cancer-suppressing agent comprising a novel cancer-suppressing gene based on the discovery of such cancer-suppressing gene. The present invention provides A cancer-suppressing agent which comprises PCDH20 gene or a homologous gene thereof.

Growing community of inventors

Tokyo, Japan

Johji Inazawa