Arbor Vita Corporation

Becton Dickinson and Company Limited

Johannes Schweizer

Peter S Lu

Michael P Belmares

Chamorro Somoza Diaz-Sarmiento

Joshua D Rabinowitz

Jonathan David Garman

John D Mantlo

Stephen J Lovell

Virginia M Crews

Lydia Blank

Nancy Hasse

Peter S Arbor Vita Corporation Lu

Methods for producing a protein extract from cells, such as cells containing viral proteins, are provided. In general terms, the methods involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent, neutralizing the pH of the intermediate composition to produce the protein extract. Kits and compositions for practicing the subject methods are also provided.

Method of protein extraction from cells

The subject invention provides antibodies, including polyclonal and monoclonal antibodies, that bind to E6 proteins from at least three oncogenic strains of HPV. In general, the antibodies bind to amino acids motifs that are conserved between the E6 proteins of different HPV strains, particularly HPV strains 16 and 18. The subject antibodies may be used to detect HPV E6 protein in a sample, and, accordingly, the antibodies find use in a variety of diagnostic applications, including methods of diagnosing cancer. Kits for performing the subject methods and containing the subject antibodies are also provided.

Antibodies for oncogenic strains of HPV and methods of their use

The invention provides reagents and methods for detecting pathogen infections in human samples. This detection utilizes specific proteins to detect the presence of pathogen proteins or abnormal expression of human proteins resulting from pathogen infections. Specific methods, compositions and kits are disclosed herein for the detection of oncogenic Human papillomavirus E6 proteins in clinical samples.

Systems of diagnosing cervical cancer

Methods of diagnosing cervical cancer

The invention provides reagents and methods for inhibiting or enhancing interactions between proteins in hematopoietic cells and other cells involved in the mediation of an immune response. Reagents and methods provided are useful for treatment of a variety of diseases and conditions mediated by immune system cells.

Arrays of PDZ domain polypeptides

Methods of identifying modulators of interaction between PDZ domain proteins and PL proteins

The invention provides reagents and methods for detecting pathogen infections in human samples. This detection utilizes specific proteins to detect the presence of pathogen proteins or abnormal expression of human proteins resulting from pathogen infections. Specific methods, compositions and kits are disclosed herein for the detection of oncogenic HumanE6 proteins in clinical samples.

Methods are provided for determining interactions between multiple PDZ-domain polypeptides and PDZ Ligand Proteins.

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Mountain View, CA, United States of America

Johannes Schweizer