Roche Diagnostics Operations, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Johannes Polz

Barbara Upmeier

Toralf Zarnt

Dieter Roessler

A method for determining an analyte in a sample suspected to comprise the analyte, includes a) contacting with said sample at least a first and a second capture compound for the analyte; b) contacting the capture compounds contacted with the sample with a specifier; c) determining the amount of complexes comprising the specifier and a capture compound; and d) determining the analyte in a sample based on the result of step c). Additionally, a method for improving the specificity of an indirect immunoassay for determining an analyte, kits, devices, and uses related to the aforementioned methods are also disclosed.

Methods for reducing interferences

Disclosed is an immunoassay method for detecting an analyte such as an antigen or an antibody in an isolated sample suspected to contain the analyte by incubating the sample with a plurality of binding partners, one of which carries a detectable label, wherein a label-specific binding partner is added that does not carry a label but binds to the detectable label. The method is applicable for a large variety of analytes and has proven particularly useful for analyte antibodies of the IgG and IgM class present in samples due to infections by pathogens. Also disclosed is a reagent kit useful for the method comprising at least two analyte-specific binding partners one of which carries a detectable label and a label-specific binding partner that binds to said detectable label but itself does not carry a detectable label.

Method for reduction of interferences in immunoassays

Described is a method for determining an analyte in a sample suspected to contain the analyte, by a) contacting the sample with a first and a second capture compound for the analyte, wherein the first and second capture compounds are non-identical capture compounds, and the capture compounds compete in binding to the analyte; b) contacting the capture compounds contacted with the sample with a specifier, wherein the specifier competes in binding to the capture compounds with the analyte; c) determining the amount of complexes having the specifier and a capture compound; and d) determining the analyte in a sample based on the result of step c). Also disclosed is a method for improving the specificity of an indirect immunoassay for determining an analyte, as well as kits, devices, and uses related to the methods.

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Murnau, Germany

Johannes Polz