Novogy, Inc.

Dsm IP Assets B.v.

Dartmouth College

Lallemand Hungary Liquidity Management LLC

Enchi Corporation

Ut-Battelle, Inc.

Technische Universiteit Delft

Tate & Lyle Ingredients Americas LLC

Ginkgo Bioworks, Inc.

Johannes Pieter Van Dijken

Jacobus Thomas Pronk

Nicky Caiazza

Arthur J Shaw, Iv

Aaron Adriaan Winkler

Vineet Rajgarhia

John E McBride

Allan C Froehlich

Elena E Brevnova

Shital A Tripathi

Colin Robert South

William Ryan Sillers

Sipko Maarten Kuyper

Aaron Argyros

Trisha Barrett

Antonius Jeroen Adriaan Van Maris

Adam Michael Guss

Wilhelmus Theodorus Antonius Maria De Laat

James H Flatt

Daniel G Olson

Christopher D Herring

Arthur J Shaw

Frank Agbogbo

Vasiliki Tsakraklides

Hendrik Wouter Wisselink

D Aaron Argyros

Jonathan Friedlander

Annapurna Kamineni

Maureen Hamilton

Wilhelmus Theordorus Antonius Maria De Laat

Johannes Hendrik De Winde

Jonathan Lo

Yu Deng

Stephen R Rogers

Johannes Hendrik Winde

Rintze Meindert Zelle

Antonius Jeroen Adriaan Maris Van

Johannes Hendrik Winde De

Adriaan Winkler Aaron

The present invention provides for novel metabolic pathways leading to propanol, alcohol or polyol formation in a consolidated bioprocessing system (CBP), where lignocellulosic biomass is efficiently converted to such products. More specifically, the invention provides for a recombinant microorganism, where the microorganism expresses one or more native and/or heterologous enzymes; where the one or more enzymes function in one or more engineered metabolic pathways to achieve: (1) conversion of a carbohydrate source to 1,2-propanediol, isopropropanol, ethanol and/or glycerol; (2) conversion of a carbohydrate source to n-propanol and isopropanol; (3) conversion of a carbohydrate source to isopropanol and methanol; or (4) conversion of a carbohydrate source to propanediol and acetone; wherein the one or more native and/or heterologous enzymes is activated, upregulated or downregulated.

Production of propanols, alcohols, and polyols in consolidated bioprocessing organisms

Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical phosphorus or sulfur sources. Fermentation methods using the genetically engineered organisms are also described. The fermentation methods are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, an pharmaceuticals.

Microorganisms engineered to use unconventional sources of phosphorous or sulfur

The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.

Engineering an increase in ethanol production by altering cofactor specificity

Disclosed are transformed cells comprising one or more genetic modifications that increase the lipid content of the cell, e.g., relative to an unmodified cell of the same type. Also disclosed are methods for increasing the lipid content of a cell by increasing the activity of one or more proteins in the cell and/or by decreasing the activity of one or more proteins in the cell.

Enhanced production of core lipids in oleaginous yeasts

Disclosed are nucleotide sequences and corresponding amino acid sequences ofgenes that can be utilized to manipulate the lipid content and/or composition of a cell. Methods and compositions for utilizing this information are disclosed to increase the lipid content or modify the lipid composition of a cell by either increasing or decreasing the activity of certain genetic targets.

Increasing lipid production and optimizing lipid composition

Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical phosphorus or sulfur sources. Fermentation methods using the genetically engineered organisms are also described. The fermentation methods are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals.

The present invention provides for the manipulation of carbon flux in a recombinant host cell to increase the formation of desirable products. The invention relates to cellulose-digesting organisms that have been genetically modified to allow the production of ethanol at a high yield by redirecting carbon flux at key steps of central metabolism.

Engineering microorganisms to increase ethanol production by metabolic redirection

The invention relates to an eukaryotic cell expressing nucleotide sequences encoding the ara A, ara B and ara D enzymes whereby the expression of these nucleotide sequences confers on the cell the ability to use L-arabinose and/or convert L-arabinose into L-ribulose, and/or xylulose 5-phosphate and/or into a desired fermentation product such as ethanol. Optionally, the eukaryotic cell is also able to convert xylose into ethanol.

Metabolic engineering of arabinose-fermenting yeast cells

The present invention relates to genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers on the host cell the ability to isomerize xylose to xylulose. These genetic modifications are aimed at improving the efficiency of xylose metabolism and include. e.g., reduction of nonspecific aldose reductase activity, increased xylulose kinase activity and increased flux of the pentose phosphate pathway. The modified host cells of the invention are suitable for the production of a wide variety of fermentation products, including ethanol, in fermentation processes in which a source of xylose or a source of xylose and glucose are used as carbon source.

Metabolic engineering of xylose-fermenting eukaryotic cells

The present invention relates to further genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers the host cell the ability of isomerizing xylose to xylulose. The further genetic modifications are aimed at improving the efficiency of xylose metabolism and include e.g. reduction of unspecific aldose reductase activity, increased xylulose kinase activity and increased flux of the pentose phosphate pathway. The modified host cells of the invention are suitable for the production of a wide variety of fermentation products, including ethanol, in fermentation processes in which a source of xylose or a source of xylose and glucose are used as carbon source.

Xylose-fermenting yeast engineered to increase flux of the pentose phosphate pathway

Disclosed herein are glucose tolerant Ccarbon source-independent (GCSI) yeast strains having no detectable amount of pyruvate decarboxylase activity, wherein wild type yeast strains for the glucose tolerant Ccarbon source-independent yeast strains are Crabtree positive. Also disclosed are methods of selecting glucose tolerant Ccarbon source-dependent yeast strains, and methods of producing pyruvic acid or salts thereof using glucose tolerant Ccarbon source-independent yeast strain. Further disclosed herein are GCSI yeast strains having a genome that comprises an exogenous lactate dehydrogenase gene.

Pyruvate producing yeast strain

Growing community of inventors

Leidschendam, Netherlands

Johannes Pieter Van Dijken