Danisco US Inc.

Dyadic Nederland B.v.

Johannes Heinrich Visser

Jacob B Visser

Peter Jan Punt

Mark Aaron Emalfarb

Richard Paul Burlingame

Jan Cornelis Verdoes

Christine Marie Pynnonen

Jan Wery

Phillip T Olson

Jean-Paul Meijnen

Jurian Alexander Bronkhof

Carola Bianca Michielse

Ismail Atalay Van Der Burgt

The present invention provides a new fungal production system comprising a fungal host strain ofwherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion ofstrain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous &#x3b2;-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion ofstrain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed. Finally a method for simplified screening of strains functionally expressing a desired enzyme by application of said strains have been provided.

Fungal production system

Provided are cells having an increased protein production characterized in that said cell comprises modified SUMOylation, a process for producing such a cell or expression system and the use of such a cell in producing a protein of interest.

Fungal high-level protein production system

Growing community of inventors

Wijchen, Netherlands

Johannes Heinrich Visser