Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Johannes Dominik Seelig

Ivan Vishniakou

A method for optimizing parameters of a physical light propagation model. The method includes making available a physical model of a light propagation in an optical system, radiating an input-light distribution into an excitation path of the optical system using an illumination unit, passing the input light distribution through a scattering body, wherein the scattering body is placed in the excitation path of the optical system so that the input-light distribution is changed to a reflection-light distribution, recording the reflection-light distribution, transferring the recorded reflection-light distribution to the physical model, and calculating distortion parameters of the physical model based on the reflection-light distribution. The distortion parameters characterize the scattering body. The reflection-light distribution is at least partially reflected as fluorescence radiation by a fluorescence-capable body within the scattering body.

Method and illumination apparatus of adaptive optics in reflection microscopy

A method for optimizing parameters of a physical light propagation model includes providing a physical model of a light propagation in an optical system, radiating an input light distribution using an illumination unit into an excitation path of the optical system, traversing the input light distribution through a scattering body, wherein the scattering body is arranged in the excitation path of the optical system and modifies the input light distribution to form a transmission light distribution to form a reflection light distribution, recording the transmission light distribution or the reflection light distribution, transferring the recorded transmission light distribution or the recorded reflection light distribution to the physical model, and computing transmission distortion parameters of the physical model based on the recorded transmission light distribution or the recorded reflection light distribution. The transmission distortion parameters characterize the scattering body.

Method and illumination device of the adaptive optics in transmission or reflection microscopy

A method for training a mathematical model which describes a light propagation in a reflection microscopy includes radiating a light distribution Iinto an excitation path of a microscope, modulating the light distribution Ito form a light distribution Iin the excitation path via an optical modulator, reflecting the light distribution Iat a location of a sample in a detection path of the microscope, modulating the light distribution Ito form a light distribution Iin the detection path via a further optical modulator, recording a reflected light distribution I, repeating the above steps n-fold to generate an n-fold 3-tuple (M, M; I), transferring the n-fold 3-tuple (M, M; I) to a computer to implement a mathematical model F for a light propagation in reflection microscopy, and ascertaining the mathematical model F which describes the light propagation in reflection microscopy based on the n-fold 3-tuple (M, M; I).

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BONN, Germany

Johannes Dominik Seelig