Basf Se Corporation

Henkel Ag & Company, Kgaa

Johannes Bongaerts

Karl-Heinz Maurer

Stefan Evers

Christian Degering

Thorsten Eggert

Thomas Weber

Timothy O'Connell

Hendrik Hellmuth

Thomas Eiting

Cornelius Bessler

Petra Siegert

Susanne Tondera

Lukas Maksym

Tobias Kueppers

Victoria Steffen

Renee Charlott Eichstadt

Ramona Knab

Renée Charlott EICHSTÄDT

Timothy O'CONNELL

Cornelius Besseler

Tobias KÜPPERS

Victoria Radnai

The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

Expression vectors with promoter and nucleic acid

The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a promoter sequence and a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

Expression vectors for an improved protein secretion

In a microbial fermentation, the aim is to increase the product yield of protein. This is achieved by a method in which an expression construct is introduced into a microorganism of the specieswhich comprises a promoter and a nucleic acid coding for the protein, and the protein is expressed in said expression construct.

Expression method

The aim is to increase the protein product yield in microbial fermentation. This is achieved by a method which introduces into a microorganism not only a first expression construct which encodes the protein, but also a second expression construct which encodes an auxiliary protease which differs from the protein, is proteolytically active and which comprises an amino acid sequence which is at least 50% identical to the amino acid sequence indicated in SEQ ID NO. 1.

In a washing or cleaning agent that contains a solid enzyme formulation, the intention is to improve shelf stability in terms of enzymatic activity. This is achieved by a washing or cleaning agent comprising an enzyme granulate, the granulate comprising, besides the enzyme, the following components: (a) 68 to 90 wt % (w/w) sulfate, in particular alkali metal sulfate, particularly preferably sodium sulfate, (b) 0.1 to 10.5 wt % (w/w) polyethylene glycol, (c) 0.5 to 14.5 wt % (w/w) granulation adjuvant.

Detergents or cleaning agents having a solid enzyme formulation

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Dormagen, Germany

Johannes Bongaerts