Curetis Gmbh

Biocartis, Nv

Johannes Bacher

Andreas Boos

Gerd Lüdke

Ronald Cornelis De Gier

Jens-Peter Seher

Adrianus Wilhelmus Dionisius Maria Van Den Bijgaart

Michiel Johannes Jongerius

Michiel De Jong

Gerhard Pross

Chris Van Haag

Danny Genius Aldegonda Schaefer

Gerd Luedke

Hassan Motejadded

The present invention provides an apparatus and a method for a lysis procedure, in particular for an automated and/or controlled lysis procedure of a sample, in particular a biological sample. The apparatus comprises at least one rotation disc (), at least one vial holder () which is configured to receive a vial (), wherein the vial holder () is arranged on the disc (), at least one driving device () which is configured to rotate the disc () and the vial holder (), at least one heating device () which is configured to heat the sample to a determined incubation temperature, and&#x2014;at least one control device () which is configured to control the driving device () and/or the heating device () by means of a timing and/or step control, and/or&#x2014;at least one transmitting device () for inductive coupling for energy and signal transmission, which is configured to transmit the energy for heating to the heating device (), and/or&#x2014;wherein the driving device () is configured to rotate the disc () in a first direction (A) and/or with a first speed, and to rotate the vial holder () in a second direction (A) and/or with a second speed. The apparatus and the method are adapted for an (automated) lysis procedure, wherein the lysis can be carried out in a safe, efficient and effective manner.

Apparatus and method for a lysis of a sample, in particular for an automated and/or controlled lysis of a sample

The present invention provides a reaction vessel () for a PCR device. The reaction vessel () comprises a sample vial () defining a reaction chamber () for performing PCR and a storage vessel () defining a storage chamber () for optical detection. The reaction chamber () is in fluid communication with a liquid supply port () for supplying a liquid sample containing at least one target DNA to the reaction chamber (). The reaction chamber () and the storage chamber () are in fluid communication via a spacer element () and a porous membrane () for hybridization of the at least one target DNA within the liquid sample onto specific immobilised hybridization probes. The lower end of the spacer element () extends into the reaction chamber (), but does not reach the bottom thereof. The upper end of the spacer element () is located in proximity of the porous membrane (), which is made from a material having different physical properties in a dry state and a wet state. In the dry state the porous membrane () allows air as well as liquid to pass therethrough. In the wet state the porous membrane () still allows the passage of liquid therethrough, but not of air, such that during a PCR the liquid sample remains in the reaction chamber () and after the PCR the reaction vessel () is configured to force the liquid sample via the spacer element () to the porous membrane () for hybridization and detection of the at least one target DNA in the liquid sample. Moreover, a PCR device comprising such a reaction vessel () as well as a method for performing PCR are described.

Reaction vessel for PCR device and method of performing PCR

Cartridge and method for the detection of the presence, absence and/or amount of a target nucleotide sequence in a sample comprising one or more nucleic acid sequences. The cartridge comprises a one or more nucleic acid sequences, and the cartridge includes a generic part and one or more separate application-specific parts, which are connectable to the generic part.

Cartridge, system and method for automated medical diagnostics

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Leonberg-Warmbronn, Germany

Johannes Bacher