Boehringer Ingelheim Vetmedica Gmbh

Stichting Centraal Diergeneeskundig Instituut

Stichting Dienst Landbouwkundig Onderzoek

Johanna Jacoba Maria Meulenberg

Robertus Jacobus Maria Moormann

Petrus Antonius Van Rijn

Gert Wensvoort

Catharinus Terpstra

Joannes Maria Anthonis Pol

Johannes Maria Antonius Pol

Judy Norma Aletta Bos-de Ruijter

Monique Helene Verheije

Michiel Valentijn Kroese

The invention relates to the field of PRRS viruses and infectious clones obtained from PRRS viruses. Furthermore, the invention relates to vaccines and diagnostic assays obtainable by using and modifying such infectious clones of PRRS viruses. The invention provides a porcine reproductive and respiratory syndrome virus (PRRSV) replicon having at least some of its original PRRSV nucleic acid deleted, said replicon capable of in vivo RNA replication, said replicon further having been deprived of at least some of its original PRRSV nucleic acid and/or having been supplemented with nucleic acid derived from a heterologous microorganism.

PRRSV vaccines

An infectious clone based on the genome of a wild-type RNA virus is produced by the process of providing a host cell not susceptible to infection by the wild-type RNA virus, providing a recombinant nucleic acid based on the genome of the wild-type RNA virus, transfecting the host cell with the recombinant nucleic acid and selecting for infectious clones. The recombinant nucleic acid comprises at least one full-length DNA copy or in vitro-transcribed RNA copy or a derivative of either. The infectious clones can be used in single or dual purpose vaccines and in viral vector vaccines.

Infectious clones of RNA viruses and vaccines and diagnostic assays derived thereof

Composition of matter comprising the causative agent of Mystery Swine Disease, Lelystad Agent, in a live, attenuated, dead, or recombinant form, or a part or component of it. Vaccine compositions and diagnostic kits based thereon. Recombinant nucleic acid comprising a Lelystad Agent-specific nucleotide sequence. Peptides comprising a Lelystad Agent-specific amino acid sequence. Lelystad Agent-specific antibodies.

Causative agent of the Mystery Swine Disease, vaccine compositions and diagnostic kits

Causative agent of the mystery swine disease, vaccine compositions and diagnostic kits

Mystery swine disease diagnostic kits

Growing community of inventors

Amsterdam, Netherlands

Johanna Jacoba Maria Meulenberg