Valtion Teknillinen Tutkimuskeskus

Teknologian Tutkimuskeskus Vtt

Rohm Enzyme Finland Oy

Alko Ltd.

Johanna Buchert

Liisa Viikari

Matti Siika-Aho

Merja Elisa Penttila

Markku Saloheimo

Hanna Kontkanen

Anu Saloheimo

Marjatta Ranua

Ann Westerholm-Parvinen

Raija Lantto

Tapani Vuorinen

Tiina Nakari-Setälä

Kristiina Kruus

Mari Hakkinen

Karin Autio

Maija Tenkanen

Marjaana Rättö

Michael Bailey

Anita Teleman

Ville Pihlajaniemi

Pasi Halonen

Emilia Selinheimo

Antti Nyyssola

Christa Schönberg

Marja-Leena Niku-Paavola

Elisabeth Heine

The present invention relates to novel polypeptides, or fragments of polypeptides, genes encoding them and means for producing said polypeptides. In detail the invention relates to polypeptides having esterase, suberinase and/or cutinase activity at low pH. This invention relates also to compositions containing the polypeptides and methods of using the polypeptides.

Polypeptides and active fragments of polypeptides having at least one esterase activity

The present invention relates to polyesterases having cutinase and/or suberinase activity obtainable fromand. The invention further relates to a method for producing the polyesterases, and to polynucleotides, vectors and host cells used therein. The enzymes are useful in hydrolysing cutin, suberin and other polyesters for example in treating agricultural or food raw materials, or wood raw materials, pulp and paper products and waste, and in modifying polyester fibers, or in laundry and dish applications.

Esterases and their use

Extracellular tyrosinases obtainable fromspp. and methods for producing them by recombinant technology. The enzymes are particularly useful in cross-linking food proteins.

Proteins having tyrosinase activity

The present invention concerns a method for enzymatic treatment of lignocellulosic materials which contain xylan-polymers, such as cellulose kraft pulps. According to a method of the present kind, at least a part of the hexenuronic acid groups present in the material is selectively removed in order to remove metal ions from the pulp, to change the surface charge thereof, to improve the brightness stability of the pulp and to render the material more suitable for enzymatic treatment.

Method of modifying a xylan-containing carbohydrate substrate having hexenuronic acid groups attached to the xylan

The present invention relates to a DNA sequence, which codes for endomannanase produced by fungi of the genus Trichoderma and transferred into a yeast or fungus strain induces that strain to produce endomannanase, as well as to a method for isolating genes coding for endomannanases. The invention also relates to vectors, yeast strains and fungal strains containing the DNA sequence. Furthermore, the invention provides an enzyme product containing at least one endomannanase, which contains at least one of the following endomannanases produced by fungi of the Trichoderma genus: an enzyme having mannanase activity and an isoelectric point (pI) of about 3.8, an enzyme having mannanase activity and a pI of about 4.1, an enzyme having mannanase activity and a pI of about 4.5, an enzyme having mannanase activity and a pI of about 5.4 and an enzyme having mannanase activity and a pI of about 6.5, the isoelectric points being determined by isoelectric focusing. The endomannanase enzyme and enzyme products according to the invention can be used for hydrolyzation of mannopolymers, in particular in connection with bleaching of lignocellulosic pulps.

Mannanase enzymes, genes coding for them and a method for isolating the

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Espoo, Finland

Johanna Buchert