Biometra Biomedizinische Analytik Gmbh

Institut Fuer Physikalische Hochtechnologie E.v.

Institut Fur Physikalische Hochtechnologie E.v

Genetrix B.v.

Johann Michael Köhler

Siegfried Poser

Ulrich Bodner

Dieter Schimkat

Ulrich Dillner

Volker Baier

Torsten Schulz

Frederik Schut

Wolfgang Fritzsche

Andreas Mokansky

Paris Som Tjwan Tan

Andrea Czaki

Antje Wiegand

Louis Oosting

A miniaturized temperature-zone flow reactor, used for thermally controlled biochemical or molecular-biological processes, especially polymerase chain reaction (PCR) enables more efficient reactions by providing at least one closed flow path which is divided into three partial paths (A. . . An; B. . . Bn and BB. . . BBn&#x2212;1; C. . . Cn) with the reactor having three substrate chips (A; B; C) which are made of a material having a high heat conductivity, and which have defined channel sections that are spaced apart relative to each other, and are connected by a connecting chip (V) made of a poor heat-conductive material. The substrate chips (A; B; C) are maintained at different temperatures by various means, including the use of controlling heating elements in contact with the chips.

Miniaturized temperature-zone flow reactor

The invention relates to an affinity sensor for detecting specific molecular binding events, for use in the field of molecular biology, e.g., in medical diagnostics, especially in biosensor technology or in DNA microarreay tests. The aim of the invention is to provide an affinity sensor of this type for rapidity, sensitively, specifically, economically and routinely detecting the presence of molecules, especially bioactive molecules, and to provide special applications for an affinity sensor of this type. To this end, the affinity sensor consists of a support substrate which is provided with at least two electrodes. The electrodes are situated equidistantly from each other and cover an area on both sides, at least this area being provided for receiving immobilized specific binding partners which are capable of coupling complementary corresponding binding partners directly or with other specific binding molecules. The area is established with a minimum width b, in such a way that at least one complementary corresponding binding partner which is provided with an electroconductive particle can be received in the area in such a way as to guarantee the possibility of a tunnel-type contact junction forming between the particle and the electrodes in each case. The affinity sensor is used for biomonitoring.

Affinity sensor for detecting specific molecular binding events and use thereof

Miniaturized multi-chamber thermocycler

Miniaturized flow thermocycler

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Golmsdorf, Germany

Johann Michael Köhler