Boehringer Mannheim Gmbh

Johann Mattersberger

Rainer Wehner

Rolf Deeg

Udo Becker

Gerd Kleinhammer

Harald Haug

Dagmar Klenner

Gerlinde Deutsch-Weyer

The present invention provides a method for the determination of a component of an immune reaction in a plasma sample using an immunoassay at a temperature of from 15.degree. to 40.degree. C., where one of the reactive components is in solid phase. The invention involves adding an amount of plasminogen activator sufficient to eliminate interference by fibrinogen with the component to be determined.

Process for the determination of a component of an immune reaction in a

This invention teaches a process for preparing a conjugate useful in enzyme immunoassays, and the conjugate produced by the process. The process involves oxidizing an enzyme containing a carbohydrate portion, such as peroxidase, with periodic acid or an alkali metal salt thereof in an aqueous medium followed by reduction with sodium borohydride. The oxidation and reduction step may be carried out either before or after the enzyme is coupled to the immunologically effective component of the conjugate.

Conjugate for enzyme immuno determinations

The present invention provides a process for stabilizing the activity of peroxidase in solution by the addition of a specific activity stabilizer, wherein, to the enzyme present in solid or dissolved form, there is added, as activity stabilizer, phenol which optionally contains one or more substituents selected from lower alkyl radicals and chlorine and bromine atoms, in an amount of from 0.0005 to 2% by weight, referred to the solution.

Process for stabilizing the activity of peroxidase in solution

Growing community of inventors

Munich, Germany

Johann Mattersberger