Ge Healthcare Bio-sciences Ab

Cytiva Bioprocess R&d Ab

Johan William Ohman

Therese Graner

Camilla Estmer Nilsson

Mats Ander

Nils Norrman

Ronnie Palmgren

Anders Larsson

Jinyu Zou

James Van Alstine

Philippe Busson

Daniel Leif Ivansson

Ulrika Meyer

Anneli Jorsback

Klas Allmer

John Daicic

The present invention relates to the field of chromatography and more specifically to producing protein affinity chromatography resins comprising affinity ligands based on a N-terminal fragment of a split intein, such as DnaE from, as well as methods for using the same. The N-terminal fragments are produced in inclusion bodies in bacterial cells.

Chromatography resin, production and use thereof

Provided are methods for protein analysis in which proteins to be analyzed are displayed on a population of discrete and dispersible structures, such as beads or other particles, for subsequent affinity reactions and analysis/detection. The proteins to be analyzed may be provided as denatured protein samples in the presence of a denaturing agent.

Method for protein analysis

The invention relates to a system and method for the stable storage of sensitive biological or chemical target substance, in a bound form on certain capture media. The method comprised providing a sample containing the target substance in a suitable buffer; combining the sample with a capture media to effect reversible binding of the target substance to the capture media; and storing the capture media with the target substance at between about &#x2212;20 and 20&#xb0; C.; and recovering the target substance from the capture media. The target substance recovered maintains the desired activity. Also provides are methods for reducing aggregates in the sensitive biological or chemical target substance.

Method for storage and stabilization of a target substance

The present invention relates to a simple and efficient method to isolate and purify nucleic acids, preferably genomic DNA, from complex samples compared with available methods, by using a ligand which relies on hydrogen bonding to purify the nucleic acids. Preferably the ligand is bound to magnetic beads/particles. More closely the method comprises adding a sample comprising nucleic acid to a polymer having neutral charge; reversibly binding said nucleic acid to said polymer by hydrogen bonding under pH conditions &#x3c;5; washing said polymer; and eluting said nucleic acid from said polymer under conditions of pH &#x3e;5. The method is very suitable for sample preparation of nucleic acids, for example for PCR applications.

Purification of nucleic acid

The present invention relates to separation of biomolecules. More closely, the invention relates to a method for production of a separation medium comprising hybrid particles of inorganic and organic material as well as the hybrid particles produced by this method. Finally, the invention relates to use of the hybrid particles for separation of biomolecules, preferably phosphoproteins. The method comprises the following steps: addition of inorganic metal oxide particles to an organic solution to form a mixture; and emulsification of the mixture to form porous hybrid particles, wherein the density of the porous hybrid particles is between 1.0 and 1.5 g/ml, and wherein the inorganic particles have a shape and size that maximizes their active surface area enabling the inorganic particles to interact with biomolecules.

Separation media for biomolecules comprising porous hybrid particles containing inorganic metal oxide nanoparticles

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Uppsala, Sweden

Johan William Ohman