Gist-brocades N.v.

Gist-brocades B.v.

Shell Internationale Research Maatschappij, B.v.

Sanders, Johan P. M.

Linnett, Paul E.

Johan Pieter Sanders

Peter Michael Andreoli

Leo J Mulleners

Yvonne J Vos

Jan H Van Ee

Johannes Abel Van Den Berg

Andrew J Docherty

Johannes A Van Den Berg

Paul E Linnett

Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing the gene expressing the polypeptide of interest are introduced into a compatible host. Transformed hosts harboring the introduced vehicle in a stable way by integration of the vehicle into the host cells chromosome are selected. Efficient transfer of the vehicle containing the gene of interest is achieved, with the resulting industrial strain transformants being effective, stable producers of the desired polypeptide product.

Transformed industrial bacillus strains and methods for making and using

Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing the gene expressing the polypeptide of interest are introduced into a compatible host. Transformed hosts harboring the introduced vehicle in a stable way by integration of the vehicle into the host cells chromosome are selected. Efficient transfer of the vehicle containing the gene of interest is achieved with the resulting industrial strain transformants being effective, stable producers of the desired polypeptide product.

Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing the gene expressing the polypeptide of interest are introduced into a compatible host. Transformed hosts harboring the introduced vehicle in a stable way by integration of the vehicle into the host cells chromosome are selected Efficient transfer of the vehicle containing the gene of interest is achieved, with the resulting industrial strain transformants being effective, stable producers of the desired polypeptide product.

Molecular cloning and expression in industrial Bacillus species

Process for the preparation of oligosaccharides-containing products from biomass involving the use of hydrochloric acid for partly hydrolyzing biomass to easily fermentable oligosaccharides-containing products and easy recovery of the hydrochloric acid and the oligosaccharides containing products. The oligosaccharides-containing products themselves and use of the latter for the preparation of useful products like ethanol and penicillin by fermentation.

Process for the preparation of oligosaccharides-containing products from

Plasmid conferring resistance to streptomycin (Sm.sup.R) and neomycin (Neo.sup.R) upon its host (e.g. a Bacillus, Staphylococcus or Escherichia coli) and which after having taken up a foreign DNA fragment at one of its restriction sites with loss of its Sm.sup.R or Neo.sup.R phenotype, is still capable of replicating and expressing genetic information in its host.

Growing community of inventors

Delft, Netherlands

Johan Pieter Sanders