Chemometec A/s

Johan Holm

Martin Glensbjerg

Frans Ejner Ravn Hansen

Søren Kjaerulff

Søren Kjærulff

Frans Ejner Ravn

A method for characterizing object(s) in a sample includes collecting transmitted, refracted, scattered, diffracted, and/or emitted light from the sample. The collected light formed on a sensor surface includes at least a first and second image of the object(s). For at least the first and second image, the collected light is modulated asymmetrically differently, or for at least a third and fourth image focal plane positions are different, or for at least a fifth image the collected light is modulated in at least two places differently compared to the surroundings, or for at least a sixth and seventh image of the object(s), the collected light is modulated asymmetrically differently and focal planes of the sixth and seventh image have different positions. The images are processed to characterize the objects(s).

Depicting of objects

The present invention relates to methods and systems for image cytometry analysis, typically at low optical magnification, where analysis is based on detection of biological particles using UV bright field and optionally one or more sources of excitation light.

Image forming cytometer

The present invention relates to methods and systems for image cytometry analysis, in particular using light sources to be cooled. Thereby is provided optimal light conditions for image cytometry.

Image cytometer implementation

The present invention relates to methods and systems for image cytometry analysis, typically at low optical magnification, where analysis is based on detection of biological particles using UV bright field, dark field or one or more sources of excitation light. The system comprises illumination means (), a sample holder (), a sample compartment (), imaging means (), collection means (), light modulation means (), and detection means () with active detection elements ().

The invention relates to image analysis of dark field images obtained at low magnification below 10:1. Image analysis of dark field images obtained at low magnification can be combined with analyzes of images obtained in respect of the same section of a sample and same magnification but with other techniques such as fluorescent microscopy. The system and method can be used e.g. for particle counting, particle size measurement, particle size distribution, morphology measurement, where the particles can be cells and/or cell parts. The invention also relates to a compact dark field light source unit, a system or apparatus including a microscope which by itself is compact and comprises the mentioned dark field light source unit.

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Søborg, Denmark

Johan Holm